Sangili, A, Veerakumar P, Chen S-M, Rajkumar C, Lin K-C.
2019.
Voltammetric determination of vitamin B2 by using a highly porous carbon electrode modified with palladium-copper nanoparticles. Microchimica Acta. 186(5):299.
AbstractPalladium-copper nanoparticles were placed on activated carbon to give a nanocomposite for electrochemical sensing of riboflavin (vitamin B 2 ). The activated carbon was produced by pyrolysis of natural waste of pistachio nutshells after KOH activation and under a nitrogen atmosphere. The carbons possess a large surface area and micro/meso-porosity. The nanocomposite was characterized by a variety of techniques to confirm structures and morphology. A screen-printed electrode modified with the composite was examined by EIS, CV, DPV, and amperometry. The effects of pH value, scan rate, and stability of the modified electrode were studied. Under optimized conditions, vitamin B 2 displays a well-expressed oxidation peak at −0.15 V (vs. Ag/AgCl) in solutions with a pH value of 7.0. The voltammetric signal increases linearly in the 0.02 to 9 μM concentrations range and a lower detection limit of 7.6 pM. The sensor was successfully applied to the determination of vitamin B 2 even in the presence of other common vitamins and in (spiked) raw milk samples. [Figure not available: see fulltext.]. © 2019, Springer-Verlag GmbH Austria, part of Springer Nature.
Srinivasan, V, Jhonsi MA, Lin K-C, Ananth DA, Sivasudha T, Narayanaswamy R, Kathiravan A.
2019.
Pyrene-based prospective biomaterial: In vitro bioimaging, protein binding studies and detection of bilirubin and Fe3+. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 211
AbstractHerein, we have meticulously derived the nanosized fluorescent aggregates from pyrene Schiff base (PS) in DMSO:water (10:90) ratio. The aggregation property of PS molecule was characterized by SEM and TEM measurements, revealed the aggregated particles are in spherical shape with ~3 nm in size. Moreover, aggregates exhibit a high fluorescence quantum yield (48%) which was effectively used for the in vitro bioimaging of two different cancer cells such as A549 and MCF-7 cells in which it exhibiting excellent biocompatibility. Further, it was estimated the capability of twofold acridine orange/ethidium bromide (AO/EB) staining to identify the apoptotic associated changes in cancer cells. Additionally, the aggregates were successfully demonstrated as a luminescent probe for the perceptive biomolecule detection of bilirubin. On the other hand, the PS molecule was successfully utilized for protein binding and metal ion sensing studies. The interaction of bovine serum albumin (BSA) with PS molecule in DMSO was using fluorescence spectroscopic method and nature of interaction was also confirmed through molecular docking analysis. The PS molecule also acts as an excellent sensor for biologically important Fe3+ ion with detection limit of 336 nM. Overall, PS molecule can be a prospective material in biological field both in solution as well as aggregated forms. © 2019 Elsevier B.V.
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