<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kao, W. C.</style></author><author><style face="normal" font="default" size="100%">Wang, V. C. C.</style></author><author><style face="normal" font="default" size="100%">Huang, Y. C.</style></author><author><style face="normal" font="default" size="100%">Yu, S. S. F.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Chan, S. I.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation, purification and characterization of hemerythrin from Methylococcus capsulatus (Bath)</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Inorganic Biochemistry</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Inorg Biochem</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bacterial chemotaxis protein</style></keyword><keyword><style  face="normal" font="default" size="100%">domain</style></keyword><keyword><style  face="normal" font="default" size="100%">electron paramagnetic resonance</style></keyword><keyword><style  face="normal" font="default" size="100%">hemerythrin</style></keyword><keyword><style  face="normal" font="default" size="100%">methane monooxygenase</style></keyword><keyword><style  face="normal" font="default" size="100%">methylococcus capsulatus (bath)</style></keyword><keyword><style  face="normal" font="default" size="100%">multiple sequence alignment</style></keyword><keyword><style  face="normal" font="default" size="100%">particulate methane monooxygenase</style></keyword><keyword><style  face="normal" font="default" size="100%">protein purification</style></keyword><keyword><style  face="normal" font="default" size="100%">resonance raman spectroscopy</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Aug</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000258012000007</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">8</style></number><volume><style face="normal" font="default" size="100%">102</style></volume><pages><style face="normal" font="default" size="100%">1607-1614</style></pages><isbn><style face="normal" font="default" size="100%">0162-0134</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Earlier work from our laboratory has indicated that a hemerythrin-like protein was over-produced together with the particulate methane monooxygenase (pMMO) when Methylococcus capsulatus (Bath) was grown under high copper concentrations. A homologue of hemerythrin had not previously been found in any prokaryote. To confirm its identity as a hemerythrin, we have isolated and purified this protein by ion-exchange, gel-filtration and hydrophobic interaction chromatography, and characterized it by mass spectrometry, UV-visible, CD, EPR and resonance Raman spectroscopy. On the basis of biophysical and multiple sequence alignment analysis, the protein isolated from M. capsulatus (Bath) is in accord with hemerythrins previously reported from higher organisms. Determination of the Fe content in conjunction with molecular-weight estimation and mass analysis indicates that the native hemerythrin in M. capsulatus (Bath) is a monomer with molecular mass 14.8 kDa, in contrast to hemerythrins from other eukaryotic organisms, where they typically exist as a tetramer or higher oligomers. (c) 2008 Elsevier Inc. All rights reserved.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000258012000007</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;331KVTimes Cited:13Cited References Count:23&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chan, SIAcad Sinica, Inst Chem, Taipei 115, TaiwanAcad Sinica, Inst Chem, Taipei 115, TaiwanAcad Sinica, Inst Chem, Taipei 115, TaiwanNatl Taiwan Univ, Dept Chem, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, Taiwan</style></auth-address></record></records></xml>