<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Detection of G-quadruplexes in cells and investigation of G-quadruplex structure of d(T2AG3)4 in K+ solution by a carbazole derivative: BMVC</style></title><secondary-title><style face="normal" font="default" size="100%">Methods Mol Biol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*G-Quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">Adenocarcinoma/genetics/pathology</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbazoles/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Computer Simulation</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA/*chemistry/ultrastructure</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescent Dyes</style></keyword><keyword><style  face="normal" font="default" size="100%">Guanine/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Lung Neoplasms/genetics/pathology</style></keyword><keyword><style  face="normal" font="default" size="100%">Potassium/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Pyridinium Compounds/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Solutions</style></keyword><keyword><style  face="normal" font="default" size="100%">Telomere/*chemistry/ultrastructure</style></keyword><keyword><style  face="normal" font="default" size="100%">Tumor Cells, Cultured</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/20012423</style></url></web-urls></urls><edition><style face="normal" font="default" size="100%">2009/12/17</style></edition><volume><style face="normal" font="default" size="100%">608</style></volume><pages><style face="normal" font="default" size="100%">183-206</style></pages><isbn><style face="normal" font="default" size="100%">1940-6029 (Electronic)1064-3745 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Verification of the existence of quadruplex structure in native human telomeres and determination of the major structure of d(T(2)AG(3))(4) (H24) in K(+) solution are the major questions regarding the structure of human telomeres. We have synthesized a fluorescent probe of 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC) that has a very high binding affinity for G-quadruplex H24. BMVC stabilizes quadruplex structures and acts as a sensitive probe to the local environment. Although the circular dichroism patterns of H24 are different in Na(+) and K(+) solutions, similar binding behaviors of BMVC to H24 in these solutions led us to suggest that the major G-quadruplex structure of H24 in K(+) solution is very likely similar to that in Na(+) solution. Of particular interest is the fluorescent band detected at -575 nm in quadruplex H24 and at -545 nm in duplex DNA. In addition, the intensity of BMVC fluorescence increases by two orders of magnitudes upon interaction with either duplex or G-quadruplex DNA. BMVC has a greater binding preference for G-quadruplex H24 than for duplex DNA. Analyzing the BMVC fluorescence at the ends of metaphase chromosomes and other regions of chromosomes allowed us to verify the presence of G-quadruplex structure in human telomeres for the first time. Using fluorescence lifetime imaging microscopy, the longer decay time of BMVC in G-quadruplex H24 than in duplex DNA allowed us to map the G-quadruplex structure in human metaphase chromosomes.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Research Support, Non-U.S. Gov't</style></work-type><accession-num><style face="normal" font="default" size="100%">20012423</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Chang, Ta-ChauChang, Cheng-ChungClifton, N.J.Methods Mol Biol. 2010;608:183-206. doi: 10.1007/978-1-59745-363-9_12.&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Institute of Atomic and Molecular Sciences, and Genomic Research Center, Academia Sinica, Taipei, Taiwan, Republic of China.</style></auth-address></record></records></xml>