<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tsai, Y. L.</style></author><author><style face="normal" font="default" size="100%">Wang, Z. F.</style></author><author><style face="normal" font="default" size="100%">Chen, W. W.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Emulsified BMVC derivative induced filtration for G-quadruplex DNA structural separation</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic Acids Res</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nucleic acids research</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*G-Quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbazoles/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA/*chemistry/*isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Emulsions</style></keyword><keyword><style  face="normal" font="default" size="100%">Filtration/*methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescent Dyes/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrocarbons, Brominated/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Microscopy, Fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">Potassium/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Pyridinium Compounds/*chemistry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Sep 1</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/21715373</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">17</style></number><edition><style face="normal" font="default" size="100%">2011/07/01</style></edition><volume><style face="normal" font="default" size="100%">39</style></volume><pages><style face="normal" font="default" size="100%">e114</style></pages><isbn><style face="normal" font="default" size="100%">1362-4962 (Electronic)0305-1048 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A novel method based on emulsion/filtration is introduced for G-quadruplex DNA structural separation. We first synthesized a lipophilic analogue of BMVC, 3,6-Bis(1-methyl-4-vinylpyridinium)-9-(12'-bromododecyl) carbazole diiodide (BMVC-12C-Br), which can form an oil-in-water (o/w) phase emulsion. Due to the binding preferences of BMVC-12C-Br emulsion to some specific DNA structures, the large emulsion ( approximately 2 microm) bound DNA was separated from the small free DNA in the filtrate by a 0.22 microm pore size MCE membrane. This method is able to isolate the non-parallel G-quadruplexes from the parallel G-quadruplexes and the linear duplexes from both G-quadruplexes. In addition, this method allows us not only to determine the absence of the parallel G-quadruplexes of d(T(2)AG(3))(4) and the presence of the parallel G-quadruplexes of d(T(2)AG(3))(2) in K(+) solution, but also to verify structural conversion from antiparallel to parallel G-quadruplexes of d[AG(3)(T(2)AG(3))(3)] in K(+) solution under molecular PEG condition. Moreover, this emulsion can separate the non-parallel G-quadruplexes of d(G(3)CGCG(3)AGGAAG(5)CG(3)) monomer from the parallel G-quadruplexes of its dimer in K(+) solution. Together with NMR spectra, one can simplify the spectra for both the free DNA and the bound DNA to establish a spectrum-structure correlation for further structural analysis.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Research Support, Non-U.S. Gov't</style></work-type><accession-num><style face="normal" font="default" size="100%">21715373</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Tsai, Yu-LinWang, Zi-FuChen, Wei-WenChang, Ta-ChauEnglandNucleic Acids Res. 2011 Sep 1;39(17):e114. doi: 10.1093/nar/gkr499. Epub 2011 Jun 28.&lt;/p&gt;
</style></notes><custom2><style face="normal" font="default" size="100%">3177220</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Chemistry, National Taiwan University, Taipei, Taiwan.</style></auth-address></record></records></xml>