<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Li PT</style></author><author><style face="normal" font="default" size="100%">Wang ZF</style></author><author><style face="normal" font="default" size="100%">Chu IT</style></author><author><style face="normal" font="default" size="100%">Kuan YM</style></author><author><style face="normal" font="default" size="100%">Li MH</style></author><author><style face="normal" font="default" size="100%">Huang MC</style></author><author><style face="normal" font="default" size="100%">Chiang PC</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author><author><style face="normal" font="default" size="100%">Chen CT</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Expression of the human telomerase reverse transcriptase gene is modulated by quadruplex formation in its first exon due to DNA methylation</style></title><secondary-title><style face="normal" font="default" size="100%">J Biol Chem.20859-2087</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2017</style></year></dates><volume><style face="normal" font="default" size="100%">292</style></volume><pages><style face="normal" font="default" size="100%">20859-20870</style></pages><issue><style face="normal" font="default" size="100%">51</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wang ZF</style></author><author><style face="normal" font="default" size="100%">Li MH</style></author><author><style face="normal" font="default" size="100%">Chen WW</style></author><author><style face="normal" font="default" size="100%">Hsu ST</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A novel transition pathway of ligand-induced topological conversion from hybrid forms to parallel forms of human telomeric G-quadruplexes.</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic Acids Res.</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2016</style></year></dates><volume><style face="normal" font="default" size="100%">44</style></volume><pages><style face="normal" font="default" size="100%">3958-68</style></pages><issue><style face="normal" font="default" size="100%">8</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chen WW</style></author><author><style face="normal" font="default" size="100%">Yi YH</style></author><author><style face="normal" font="default" size="100%">Chien CH</style></author><author><style face="normal" font="default" size="100%">Hsiung KC</style></author><author><style face="normal" font="default" size="100%">Ma TH</style></author><author><style face="normal" font="default" size="100%">Lin YC</style></author><author><style face="normal" font="default" size="100%">Lo SJ</style></author><author><style face="normal" font="default" size="100%">Chang TC.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Specific polyunsaturated fatty acids modulate lipid delivery and oocyte development in C. elegans revealed by molecular-selective label-free imaging.</style></title><secondary-title><style face="normal" font="default" size="100%">Sci Rep.</style></secondary-title></titles><dates><year><style  face="normal" font="default" 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size="100%">Wang ZF</style></author><author><style face="normal" font="default" size="100%">Wang CL</style></author><author><style face="normal" font="default" size="100%">Hsu TN</style></author><author><style face="normal" font="default" size="100%">Li PT</style></author><author><style face="normal" font="default" size="100%">Chen CT</style></author><author><style face="normal" font="default" size="100%">Lin JJ</style></author><author><style face="normal" font="default" size="100%">Lou PJ</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Direct evidence of mitochondrial G-quadruplex DNA by using fluorescent anti-cancer agents</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic Acids Res.</style></secondary-title></titles><dates><year><style  face="normal" font="default" 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face="normal" font="default" size="100%">Curr Top Med Chem.</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2015</style></year></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">1964-70</style></pages><issue><style face="normal" font="default" size="100%">19</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Lin IT</style></author><author><style face="normal" font="default" size="100%">Tsai YL</style></author><author><style face="normal" font="default" size="100%">Kang CC</style></author><author><style face="normal" font="default" size="100%">Huang WC</style></author><author><style face="normal" font="default" size="100%">Wang CL</style></author><author><style face="normal" font="default" size="100%">Lin MY</style></author><author><style face="normal" font="default" size="100%">Lou PJ</style></author><author><style face="normal" font="default" size="100%">Shih JY</style></author><author><style face="normal" font="default" size="100%">Wang HC</style></author><author><style face="normal" font="default" size="100%">Wu HD</style></author><author><style face="normal" font="default" size="100%">Tsai TH</style></author><author><style face="normal" font="default" size="100%">Jan ISang</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">BMVC test, an improved fluorescence assay for detection of malignant pleural effusions</style></title><secondary-title><style face="normal" font="default" size="100%">Cancer medicine</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style 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size="100%">PloS one</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">e86143</style></pages><issue><style face="normal" font="default" size="100%">1</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wang JM</style></author><author><style face="normal" font="default" size="100%">Huang FC</style></author><author><style face="normal" font="default" size="100%">Kuo MH</style></author><author><style face="normal" font="default" size="100%">Wang ZF</style></author><author><style face="normal" font="default" size="100%">Tseng TY</style></author><author><style face="normal" font="default" size="100%">Chang LC</style></author><author><style face="normal" font="default" size="100%">Yen SJ</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author><author><style face="normal" font="default" size="100%">Lin JJ</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Inhibition of Cancer Cell Migration and Invasion through Suppressing the Wnt1-mediating Signal Pathway by G-quadruplex Structure Stabilizers</style></title><secondary-title><style face="normal" font="default" size="100%">The Journal of biological chemistry</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Yi YH</style></author><author><style face="normal" font="default" size="100%">Chien CH</style></author><author><style face="normal" font="default" size="100%">Chen WW</style></author><author><style face="normal" font="default" size="100%">Ma TH</style></author><author><style face="normal" font="default" size="100%">Liu KY</style></author><author><style face="normal" font="default" size="100%">Chang YS</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author><author><style face="normal" font="default" size="100%">Lo SJ</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Lipid droplet pattern and nondroplet-like structure in two fat mutants of Caenorhabditis elegans revealed by coherent anti-Stokes Raman scattering microscopy.</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of biomedical optics</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23979461</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">19</style></volume><pages><style face="normal" font="default" size="100%">11011</style></pages><abstract><style face="normal" font="default" size="100%">&lt;p&gt;ABSTRACT. Lipid is an important energy source and essential component for plasma and organelle membranes in all kinds of cells. Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free and nonlinear optical technique that can be used to monitor the lipid distribution in live organisms. Here, we utilize CARS microscopy to investigate the pattern of lipid droplets in two live Caenorhabditis elegans mutants (fat-2 and fat-3). The CARS images showed a striking decrease in the size, number, and content of lipid droplets in the fat-2 mutant but a slight difference in the fat-3 mutant as compared with the wild-type worm. Moreover, a nondroplet-like structure with enhanced CARS signal was detected for the first time in the uterus of fat-2 and fat-3 mutants. In addition, transgenic fat-2 mutant expressing a GFP fusion protein of vitellogenin-2 (a yolk lipoprotein) revealed that the enhanced CARS signal colocalized with the GFP signal, which suggests that the nondroplet-like structure is primarily due to the accumulation of yolk lipoproteins. Together, this study implies that CARS microscopy is a potential tool to study the distribution of yolk lipoproteins, in addition to lipid droplets, in live animals.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wang ZF</style></author><author><style face="normal" font="default" size="100%">Li MH</style></author><author><style face="normal" font="default" size="100%">Hsu ST</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Structural basis of sodium-potassium exchange of a human telomeric DNA quadruplex without topological conversion</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic acids research,</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style face="normal" font="default" size="100%">42</style></volume><pages><style face="normal" font="default" size="100%">4723-4733</style></pages><issue><style face="normal" font="default" size="100%">7</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Li MH</style></author><author><style face="normal" font="default" size="100%">Wang ZF</style></author><author><style face="normal" font="default" size="100%">Kuo MH</style></author><author><style face="normal" font="default" size="100%">Hsu ST</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Unfolding kinetics of human telomeric g-quadruplexes studied by NMR spectroscopy</style></title><secondary-title><style face="normal" font="default" size="100%">The journal of physical chemistry. B</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style face="normal" font="default" size="100%">118</style></volume><pages><style face="normal" font="default" size="100%">931-6</style></pages><issue><style face="normal" font="default" size="100%">4</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wei, P. C.</style></author><author><style face="normal" font="default" size="100%">Wang, Z. F.</style></author><author><style face="normal" font="default" size="100%">Lo, W. T.</style></author><author><style face="normal" font="default" size="100%">Su, M. I.</style></author><author><style face="normal" font="default" size="100%">Shew, J. Y.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Lee, W. H.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic Acids Research</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nucleic Acids Res</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">c-myc</style></keyword><keyword><style  face="normal" font="default" size="100%">complex-formation</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA structures</style></keyword><keyword><style  face="normal" font="default" size="100%">gene</style></keyword><keyword><style  face="normal" font="default" size="100%">initiation</style></keyword><keyword><style  face="normal" font="default" size="100%">myc g-quadruplex</style></keyword><keyword><style  face="normal" font="default" size="100%">protein</style></keyword><keyword><style  face="normal" font="default" size="100%">region</style></keyword><keyword><style  face="normal" font="default" size="100%">telomeric DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">transcription</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Feb</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23241391</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">3</style></number><volume><style face="normal" font="default" size="100%">41</style></volume><pages><style face="normal" font="default" size="100%">1533-1543</style></pages><isbn><style face="normal" font="default" size="100%">0305-1048</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;We reported that non-targeting siRNA (NT-siRNA) stress induces non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) expression to cooperate with exoribonuclease XRN2 for releasing the stress [Wei,P.C., Lo,W.T., Su,M.I., Shew,J.Y. and Lee, W. H. (2011) Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress. Nucleic Acids Res., 40, 323-332]. However, how NT-siRNA stress inducing NPGPx expression remains elusive. In this communication, we showed that the proximal promoter of NPGPx contained a mixed G-quadruplex (G4) structure, and disrupting the structure diminished NT-siRNA induced NPGPx promoter activity. We also demonstrated that nucleolin (NCL) specifically bonded to the G4-containing sequences to replace the originally bound Sp1 at the NPGPx promoter on NT-siRNA stress. Consistently, overexpression of NCL further increased NPGPx promoter activity, whereas depletion of NCL desensitized NPGPx promoter to NT-siRNA stress. These results suggest that the cis-element with mixed G4 structure at the NPGPx promoter plays an essential role for its transactivation mediated by NCL to release cells from NT-siRNA stress.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000316351800021</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;109FMTimes Cited:0Cited References Count:33&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Lee, WHGenom Res Ctr, Taipei 11529, TaiwanGenom Res Ctr, Taipei 11529, TaiwanNatl Def Med Ctr, Grad Inst Life Sci, Taipei 11490, TaiwanGenom Res Ctr, Taipei 11529, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 11529, TaiwanNatl Taiwan Univ, Dept Chem, Taipei 11529, TaiwanAcad Sinica, Inst Biol Chem, Taipei 11529, TaiwanUniv Calif Irvine, Dept Biol Chem, Irvine, CA 92717 USA</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Meng-Chieh Hsieh</style></author><author><style face="normal" font="default" size="100%">Cheng-Hao Chien</style></author><author><style face="normal" font="default" size="100%">Cheng-Chung Chang</style></author><author><style face="normal" font="default" size="100%">Ta-Chau Chan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Aggregation induced photodynamic therapy enhancement based on linear and nonlinear excited FRET of fluorescent organic nanoparticles</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Materials Chemistry B,</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://pubs.rsc.org/en/content/articlelanding/2013/TB/c3tb00345k#!divAbstract</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">1</style></volume><pages><style face="normal" font="default" size="100%">2350-2357</style></pages><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A binary molecule can self-assemble to form fluorescent organic nanoparticles (FONs) based on the Aggregation-Induced Emission Enhancement (AIEE) property and subsequently, presents an efficient fluorescence resonance energy transfer (FRET) to generate singlet oxygen under linear and nonlinear light sources. Biologically, this FON-photosensitizer is much more phototoxic to cancer cells than to normal cells without significant dark toxicity. Eventually, a new approach, called FON FRET-PDT or AIEE FRET-PDT, to promote the PDT effect is expected.&lt;/p&gt;
</style></abstract></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Liu, S. W.</style></author><author><style face="normal" font="default" size="100%">Chu, J. F.</style></author><author><style face="normal" font="default" size="100%">Tsai, C. T.</style></author><author><style face="normal" font="default" size="100%">Fang, H. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Li, H. W.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Assaying the binding strength of G-quadruplex ligands using single-molecule TPM experiments</style></title><secondary-title><style face="normal" font="default" size="100%">Anal Biochem</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23376016</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">436</style></volume><pages><style face="normal" font="default" size="100%">101-8</style></pages><abstract><style face="normal" font="default" size="100%">&lt;p&gt;G-quadruplexes are stable secondary structures formed by Hoogsteen base pairing of guanine-rich single-stranded DNA sequences in the presence of monovalent cations (Na(+) or K(+)). Folded G-quadruplex (G4) structures in human telomeres have been proposed as a potential target for cancer therapy. In this study, we used single-molecule tethered particle motion (TPM) experiments to assay the binding strength of possible G4 ligands. We found that individual single-stranded DNA molecules containing the human telomeric sequence d[AGGG(TTAGGG)3] fluctuated between the folded and the unfolded states in a 10 mM Na(+) solution at 37 °C. The durations of folded and unfolded states were single-exponentially distributed, and in return the folding and unfolding rate constants were 1.68 ± 0.01 and 1.63 ± 0.03 (s(-1)), respectively. In the presence of G4 ligands, such as TMPyP4, DODCI, BMVC, and BMVPA, the unfolding rate constant decreased appreciably. In addition, combining the Cu(2+)-induced G4 unfolding and TPM assay, we showed that BMVC and TMPyP4 are better G4 stabilizers than DODCI. The capability of monitoring the fluctuation between the folded and the unfolded state of G4 DNA in real time allows the determination of both kinetic and thermodynamic parameters in a single measurement and offers a simple way to assay binding strength under various conditions.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chen WW</style></author><author><style face="normal" font="default" size="100%">Chien CH</style></author><author><style face="normal" font="default" size="100%">Wang CL</style></author><author><style face="normal" font="default" size="100%">Wang HH</style></author><author><style face="normal" font="default" size="100%">Wang YL</style></author><author><style face="normal" font="default" size="100%">Ding ST</style></author><author><style face="normal" font="default" size="100%">Lee TS</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Automated quantitative analysis of lipid accumulation and hydrolysis in living macrophages with label-free imaging.</style></title><secondary-title><style face="normal" font="default" size="100%">Analytical and bioanalytical chemistry</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23934396</style></url></web-urls></urls><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The accumulation of lipids in macrophages is a key factor that promotes the formation of atherosclerotic lesions. Several methods such as biochemical assays and neutral lipid staining have been used for the detection of lipids in cells. However, a method for real-time quantitative assessment of the lipid content in living macrophages has yet to be shown, particularly for its kinetic process with drugs, due to the lack of suitable tools for non-invasive chemical detection. Here we demonstrate label-free real-time monitoring of lipid droplets (LDs) in living macrophages by using coherent anti-Stokes Raman scattering (CARS) microscopy. In addition, we have established an automated image analysis method based on maximum entropy thresholding (MET) to quantify the cellular lipid content. The result of CARS image analysis shows a good correlation (R 2 &amp;gt; 0.9) with the measurement of biochemical assay. Using this method, we monitored the processes of lipid accumulation and hydrolysis in macrophages. We further characterized the effect of a lipid hydrolysis inhibitor (diethylumbelliferyl phosphate, DEUP) and determined the kinetic parameters such as the inhibition constant, K i. Our work demonstrates that the automated quantitative analysis method is useful for the studies of cellular lipid metabolism and has potential for preclinical high-throughput screening of therapeutic agents related to atherosclerosis and lipid-associated disorders.&lt;/p&gt;
</style></abstract></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kang CC</style></author><author><style face="normal" font="default" size="100%">Huang WC</style></author><author><style face="normal" font="default" size="100%">Kouh CW</style></author><author><style face="normal" font="default" size="100%">Wang ZF</style></author><author><style face="normal" font="default" size="100%">Cho CC</style></author><author><style face="normal" font="default" size="100%">Chang CC</style></author><author><style face="normal" font="default" size="100%">Wang CL</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author><author><style face="normal" font="default" size="100%">Seemann J</style></author><author><style face="normal" font="default" size="100%">Huang LJ</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Chemical principles for the design of a novel fluorescent probe with high cancer-targeting selectivity and sensitivity.</style></title><secondary-title><style face="normal" font="default" size="100%">Integrative biology : quantitative biosciences from nano to macro</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23970166</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">5</style></volume><pages><style face="normal" font="default" size="100%">1217-28</style></pages><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Understanding of principles governing selective and sensitive cancer targeting is critical for development of chemicals for cancer diagnostics and treatment. We determined the underlying mechanisms of how a novel fluorescent small organic molecule, 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC), selectively labels cancer cells but not normal cells. We show that BMVC is retained in the lysosomes of normal cells. In cancer cells, BMVC escapes lysosomal retention and localizes to the mitochondria or to the nucleus, where DNA-binding dramatically increases BMVC fluorescence intensity, allowing it to light up only cancer cells. Structure-function analyses of BMVC derivatives show that hydrogen-bonding capacity is a key determinant of lysosomal retention in normal cells, whereas lipophilicity directs these derivatives to the mitochondria or the nucleus in cancer cells. In addition, drug-resistant cancer cells preferentially retain BMVC in their lysosomes compared to drug-sensitive cancer cells, and BMVC can be released from drug-resistant lysosomes using lysosomotropic agents. Our results further our understanding of how properties of cellular organelles differ between normal and cancer cells, which can be exploited for diagnostic and/or therapeutic use. We also provide physiochemical design principles for selective targeting of small molecules to different organelles. Moreover, our results suggest that agents which can increase lysosomal membrane permeability may re-sensitize drug-resistant cancer cells to chemotherapeutic agents. &lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">10</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tseng TY</style></author><author><style face="normal" font="default" size="100%">Wang ZF</style></author><author><style face="normal" font="default" size="100%">Chien CH</style></author><author><style face="normal" font="default" size="100%">Chang TC</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In-cell optical imaging of exogenous G-quadruplex DNA by fluorogenic ligands.</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic acids research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/24030712</style></url></web-urls></urls><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Guanine-rich oligonucleotides (GROs) are promising therapeutic candidate for cancer treatment and other biomedical application. We have introduced a G-quadruplex (G4) ligand, 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide, to monitor the cellular uptake of naked GROs and map their intracellular localizations in living cells by using confocal microscopy. The GROs that form parallel G4 structures, such as PU22, T40214 and AS1411, are detected mainly in the lysosome of CL1-0 lung cancer cells after incubation for 2 h. On the contrary, the GROs that form non-parallel G4 structures, such as human telomeres (HT23) and thrombin binding aptamer (TBA), are rarely detected in the lysosome, but found mainly in the mitochondria. Moreover, the fluorescence resonant energy transfer studies of fluorophore-labeled GROs show that the parallel G4 structures can be retained in CL1-0 cells, whereas the non-parallel G4 structures are likely distorted in CL1-0 cells after cellular uptake. Of interest is that the distorted G4 structure of HT23 from the non-parallel G4 structure can reform to a probable parallel G4 structure induced by a G4 ligand in CL1-0 living cells. These findings are valuable to the design and rationale behind the possible targeted drug delivery to specific cellular organelles using GROs.&lt;/p&gt;
</style></abstract></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chou, Y. S.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Yang, T. L.</style></author><author><style face="normal" font="default" size="100%">Young, T. H.</style></author><author><style face="normal" font="default" size="100%">Lou, P. J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Photo-Induced Antitumor Effect of 3,6-Bis(1-methyl-4-vinylpyridinium) Carbazole Diiodide</style></title><secondary-title><style face="normal" font="default" size="100%">Biomed Research International</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Biomed Res Int</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bmvc</style></keyword><keyword><style  face="normal" font="default" size="100%">cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">cells</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">light</style></keyword><keyword><style  face="normal" font="default" size="100%">photodynamic therapy</style></keyword><keyword><style  face="normal" font="default" size="100%">photosensitizer</style></keyword><keyword><style  face="normal" font="default" size="100%">quadruplex structure</style></keyword><keyword><style  face="normal" font="default" size="100%">telomerase</style></keyword><keyword><style  face="normal" font="default" size="100%">tumors</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23509809</style></url></web-urls></urls><isbn><style face="normal" font="default" size="100%">2314-6133</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;We have applied a fluorescent molecule 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC) for tumor targeting and treatment. In this study, we investigated the photo-induced antitumor effect of BMVC. In vitro cell line studies showed that BMVC significantly killed TC-1 tumor cells at light dose greater than 40 J/cm(2). The fluorescence of BMVC in the tumor peaked at 3 hours and then gradually decreased to reach the control level a. er 24 hours. In vivo tumor treatment studies showed BMVC plus light irradiation (iPDT) significantly inhibited the tumor growth. At day 24 a. er tumor implantation, tumor volume was measured to be 225 +/- 79 mm(3), 2542 +/- 181 mm(3), 1533 +/- 766 mm(3), and 1317 +/- 108 mm(3) in the iPDT, control, light-only, and BMVC-only groups, respectively. Immunohistochemistry studies showed the microvascular density was significantly lower in the iPDT group. Taken together, our results demonstrated that BMVC may be a potent tumor-specific photosensitizer (PS) for PDT.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000314957000001</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;090CRTimes Cited:0Cited References Count:32&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Lou, PJNatl Taiwan Univ Hosp, Dept Otolaryngol, Taipei 10002, TaiwanNatl Taiwan Univ Hosp, Dept Otolaryngol, Taipei 10002, TaiwanNatl Taiwan Univ, Coll Med, Inst Biomed Engn, Taipei 10002, TaiwanNatl Taiwan Univ, Coll Engn, Taipei 10002, TaiwanNatl Chung Hsing Univ, Grad Inst Biomed Engn, Taichung 40254, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 10617, TaiwanNatl Taiwan Univ Hosp, Dept Otolaryngol, Taipei 10002, TaiwanColl Med, Taipei 10002, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ting-Yuan Tseng</style></author><author><style face="normal" font="default" size="100%">Cheng-Hao Chien</style></author><author><style face="normal" font="default" size="100%">Jen-Fei Chu</style></author><author><style face="normal" font="default" size="100%">Wei-Chun Huang</style></author><author><style face="normal" font="default" size="100%">Mei-Ying Lin</style></author><author><style face="normal" font="default" size="100%">Cheng-Chung Chang</style></author><author><style face="normal" font="default" size="100%">Ta-Chau Chang</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A specific fluorescent probe for visualizing G-quadruplex DNA by fluorescence lifetime imaging microscopy</style></title><secondary-title><style face="normal" font="default" size="100%">J Biomed Opt.</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23839279</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">101309</style></pages><abstract><style face="normal" font="default" size="100%">&lt;p&gt;ABSTRACT. The importance of guanine-quadruplex (G4) is not only in protecting the ends of chromosomes for human telomeres but also in regulating gene expression for several gene promoters. However, the existence of G4 structures in living cells is still in debate. A fluorescent probe, 3,6-bis(1-methyl-2-vinylpyridinium) carbazole diiodide (o-BMVC), for differentiating G4 structures from duplexes is characterized. o-BMVC has a large contrast in fluorescence decay time, binding affinity, and fluorescent intensity between G4 structures and duplexes, which makes it a good candidate for probing G4 DNA structures. The fluorescence decay time of o-BMVC upon interaction with G4 structures of telomeric G-rich sequences is ∼2.8  ns and that of interaction with the duplex structure of a calf thymus is ∼1.2  ns. By analyzing its fluorescence decay time and histogram, we were able to detect one G4 out of 1000 duplexes in vitro. Furthermore, by using fluorescence lifetime imaging microscopy, we demonstrated an innovative methodology for visualizing the localization of G4 structures as well as mapping the localization of different G4 structures in living cells. &lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">10</style></issue></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wang, Z. F.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Molecular engineering of G-quadruplex ligands based on solvent effect of polyethylene glycol</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic Acids Res</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nucleic acids research</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*G-Quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbazoles/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Circular Dichroism</style></keyword><keyword><style  face="normal" font="default" size="100%">Ligands</style></keyword><keyword><style  face="normal" font="default" size="100%">Nucleic Acid Denaturation</style></keyword><keyword><style  face="normal" font="default" size="100%">Polyethylene Glycols/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Pyridinium Compounds/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Solvents/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Telomere/*chemistry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Sep 1</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/22735707</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">17</style></number><volume><style face="normal" font="default" size="100%">40</style></volume><pages><style face="normal" font="default" size="100%">8711-20</style></pages><isbn><style face="normal" font="default" size="100%">1362-4962 (Electronic)0305-1048 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Because various non-parallel G-quadruplexes of human telomeric sequences in K+ solution can be converted to a parallel G-quadruplex by adding polyethylene glycol (PEG) as a co-solvent, we have taken advantage of this property of PEG to study the covalent attachment of a PEG unit to a G-quadruplex ligand, 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC). The hybrid ligand with the PEG unit, BMVC-8C3O or BMVC-6C2O by substituting either the tetraethylene glycol or the triethylene glycol terminated with a methyl-piperidinium cation in N-9 position of BMVC, not only induces structural change from different non-parallel G-quadruplexes to a parallel G-quadruplex but also increases the melting temperature of human telomeres in K+ solution by more than 45 degrees C. In addition, our ligand work provides further confidence that the local water structure plays the key to induce conformational change of human telomere.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">22735707</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Wang, Zi-FuChang, Ta-ChauengResearch Support, Non-U.S. Gov'tEngland2012/06/28 06:00Nucleic Acids Res. 2012 Sep 1;40(17):8711-20. Epub 2012 Jun 26.&lt;/p&gt;
</style></notes><custom2><style face="normal" font="default" size="100%">3458535</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Chemistry, National Taiwan University, Academia Sinica, Taipei 106, Taiwan, ROC.</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Huang, F. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Wang, J. M.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Lin, J. J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Induction of senescence in cancer cells by the G-quadruplex stabilizer, BMVC4, is independent of its telomerase inhibitory activity</style></title><secondary-title><style face="normal" font="default" size="100%">Br J Pharmacol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">British journal of pharmacology</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*G-Quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">Antineoplastic Agents/*pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbazoles/chemistry/*pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Aging/*drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line, Tumor</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Damage</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Regulation, Neoplastic/drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Genes, myc/genetics/physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Structure</style></keyword><keyword><style  face="normal" font="default" size="100%">Mutagenesis, Site-Directed</style></keyword><keyword><style  face="normal" font="default" size="100%">Pyrazines/chemistry/*pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Telomerase/*antagonists &amp; inhibitors/genetics/metabolism</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Sep</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/22509942</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">2</style></number><volume><style face="normal" font="default" size="100%">167</style></volume><pages><style face="normal" font="default" size="100%">393-406</style></pages><isbn><style face="normal" font="default" size="100%">1476-5381 (Electronic)0007-1188 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;BACKGROUND AND PURPOSE: Telomerase is the enzyme responsible for extending G-strand telomeric DNA and represents a promising target for treatment of neoplasia. Inhibition of telomerase can be achieved by stabilization of G-quadruplex DNA structures. Here, we characterize the cellular effects of a novel G-quadruplex stabilizing compound, 3,6-bis(4-methyl-2-vinylpyrazinium iodine) carbazole (BMVC4). EXPERIMENTAL APPROACH: The cellular effects of BMVC4 were characterized in both telomerase-positive and alternative lengthening of telomeres (ALT) cancer cells. The molecular mechanism of how BMVC4 induced senescence is also addressed. KEY RESULTS: BMVC4-treated cancer cells showed typical senescence phenotypes. BMVC4 induced senescence in both ALT and telomerase-overexpressing cells, suggesting that telomere shortening through telomerase inhibition might not be the cause for senescence. A large fraction of DNA damage foci was not localized to telomeres in BMVC4-treated cells and BMVC4 suppressed c-myc expression through stabilizing the G-quadruplex structure located at its promoter. These results indicated that the cellular targets of BMVC4 were not limited to telomeres. Further analyses showed that BMVC4 induced DNA breaks and activation of ataxia telangiectasia-mutated mediated DNA damage response pathway. CONCLUSIONS AND IMPLICATIONS: BMVC4, a G-quadruplex stabilizer, induced senescence by activation of pathways of response to DNA damage that was independent of its telomerase inhibitory activity. Thus, BMVC4 has the potential to be developed as a chemotherapeutic agent against both telomerase positive and ALT cancer cells.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">22509942</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Huang, Fong-ChunChang, Cheng-ChungWang, Jing-MinChang, Ta-ChauLin, Jing-JerengResearch Support, Non-U.S. Gov'tEngland2012/04/19 06:00Br J Pharmacol. 2012 Sep;167(2):393-406. doi: 10.1111/j.1476-5381.2012.01997.x.&lt;/p&gt;
</style></notes><custom2><style face="normal" font="default" size="100%">3481046</style></custom2><auth-address><style face="normal" font="default" size="100%">Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei, Taiwan.</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Hsieh, M. C.</style></author><author><style face="normal" font="default" size="100%">Lin, J. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Selective photodynamic therapy based on aggregation-induced emission enhancement of fluorescent organic nanoparticles</style></title><secondary-title><style face="normal" font="default" size="100%">Biomaterials</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Biomaterials</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Drug Design</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescence Resonance Energy Transfer</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Nanoparticles/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Photochemotherapy/*methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Photosensitizing Agents/chemical synthesis/chemistry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/22024361</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">3</style></number><volume><style face="normal" font="default" size="100%">33</style></volume><pages><style face="normal" font="default" size="100%">897-906</style></pages><isbn><style face="normal" font="default" size="100%">1878-5905 (Electronic)0142-9612 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Three binary molecule conjugates were designed and synthesized by conjugating a chromophore (3, 6-bis-(1-methyl-4-vinylpyridinium)-carbazole diiodide, BMVC) to mono-, bis- and trishydroxyl photosensitizers, respectively. BMVC plays the role of cancer cells recognizer; AIEE (aggregation-induced emission enhancement) generator and FRET (Fluorescence Resonance Energy Transfer) donor. The self assembling properties of these binary conjugates result in different degrees of AIEE and then achieve the formations of FONs (fluorescent organic nanoparticles), which present efficient FRET and singlet oxygen generations. Biologically, FONs-photosensitizers from these compounds were much more phototoxicities to cancer cell than to normal cell without significant dark toxicity. In addition, their intracellular fluorescent colors switching upon photo-excitation are expected to be used for further cell death biomarker applications. This improved photodynamic activity might be due to the aggregation of compounds in the cell that form FONs which can promote PDT (photodynamic therapy) and are observed in cancer cell but not normal cell.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">22024361</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Chang, Cheng-ChungHsieh, Meng-ChiehLin, Jung-ChihChang, Ta-ChauengResearch Support, Non-U.S. Gov'tEngland2011/10/26 06:00Biomaterials. 2012 Jan;33(3):897-906. doi: 10.1016/j.biomaterials.2011.10.018. Epub 2011 Oct 22.&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Graduate Institute of Biomedical Engineering, National Chung Hsing University 250, Taichung 402, Taiwan, ROC. ccchang555@dragon.nchu.edu.tw</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chien, C. H.</style></author><author><style face="normal" font="default" size="100%">Chen, W. W.</style></author><author><style face="normal" font="default" size="100%">Wu, J. T.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Investigation of lipid homeostasis in living Drosophila by coherent anti-Stokes Raman scattering microscopy</style></title><secondary-title><style face="normal" font="default" size="100%">J Biomed Opt</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Journal of biomedical optics</style></alt-title></titles><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Dec</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23208212</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">12</style></number><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">126001</style></pages><isbn><style face="normal" font="default" size="100%">1560-2281 (Electronic)1083-3668 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;To improve our understanding of lipid metabolism, Drosophila is used as a model animal, and its lipid homeostasis is monitored by coherent anti-Stokes Raman scattering microscopy. We are able to achieve in vivo imaging of larval fat body (analogous to adipose tissue in mammals) and oenocytes (analogous to hepatocytes) in Drosophila larvae at subcellular level without any labeling. By overexpressing two lipid regulatory proteins--Brummer lipase (Bmm) and lipid storage droplet-2 (Lsd-2)--we found different phenotypes and responses under fed and starved conditions. Comparing with the control larva, we observed more lipid droplet accumulation by approximately twofold in oenocytes of fat-body-Bmm-overexpressing (FB-Bmm-overexpressing) mutant under fed condition, and less lipid by approximately fourfold in oenocytes of fat-body-Lsd-2-overexpressing (FB-Lsd-2-overexpressing) mutant under starved condition. Moreover, together with reduced size of lipid droplets, the lipid content in the fat body of FB-Bmm-overexpressing mutant decreases much faster than that of the control and FB-Lsd-2-overexpressing mutant during starvation. From long-term starvation assay, we found FB-Bmm-overexpressing mutant has a shorter lifespan, which can be attributed to faster consumption of lipid in its fat body. Our results demonstrate in vivo observations of direct influences of Bmm and Lsd-2 on lipid homeostasis in Drosophila larvae.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">23208212</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Chien, Cheng-HaoChen, Wei-WenWu, June-TaiChang, Ta-ChauengResearch Support, Non-U.S. Gov't2012/12/05 06:00J Biomed Opt. 2012 Dec;17(12):126001. doi: 10.1117/1.JBO.17.12.126001.&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">National Yang-Ming University, Institute of Biophotonics, Taipei 112, Taiwan.</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Chu, J. F.</style></author><author><style face="normal" font="default" size="100%">Tsai, Y. L.</style></author><author><style face="normal" font="default" size="100%">Wang, Z. F.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Structure conversion and structure separation of G-quadruplexes investigated by carbazole derivatives</style></title><secondary-title><style face="normal" font="default" size="100%">Curr Pharm Des</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Current pharmaceutical design</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Drug Discovery</style></keyword><keyword><style  face="normal" font="default" size="100%">*G-Quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">Antineoplastic Agents/*pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbazoles/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Circular Dichroism</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Emulsions</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescence Resonance Energy Transfer</style></keyword><keyword><style  face="normal" font="default" size="100%">Ligands</style></keyword><keyword><style  face="normal" font="default" size="100%">Magnetic Resonance Spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">Micelles</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Ultrafiltration</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/22376106</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">14</style></number><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">2002-13</style></pages><isbn><style face="normal" font="default" size="100%">1873-4286 (Electronic)1381-6128 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The challenge of G-quadruplexes is that the G-rich sequences can adopt various G4 structures and possibly interconvert among them, particularly under the change of environmental conditions. Both NMR and circular dichroism (CD) show the spectral conversion of d[AG3(T2AG3)3] (HT22) from Na-form to K-form after Na+/K+ ion exchange. No appreciable change on the induced CD spectra of BMVC molecule and the single molecule tethered particle motion of HT22 in Na+ solution upon K+ titration suggests that the spectral conversion is unlikely due to the structural conversion via fully unfolded intermediate. Although a number of mechanisms were proposed for the spectral change induced by the Na+/K+ ion exchange, determining the precise structures of HT22 in K+ solution may be essential to unravel the mechanism of the structural conversion. Thus, development of a new method for separating different structures is of critical importance for further individual verification. In the second part of this review, we describe a new approach based on &quot;micelle-enhanced ultrafiltration&quot; method for DNA structural separation. The BMVC, a G-quadruplex ligand, is first modified and then forms a large size of emulsion after ultrasonic emulsification, together with its different binding affinities to various DNA structures; for the first time, we are able to separate different DNA structures after membrane filtration. Verification of the possible structural conversion and investigation of structural diversity among various G4 structures are essential for exploring their potential biological roles and for developing new anticancer drugs.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">22376106</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Chang, Ta-ChauChu, Jen-FeiTsai, Yu-LinWang, Zi-FuengResearch Support, Non-U.S. Gov'tReviewNetherlands2012/03/02 06:00Curr Pharm Des. 2012;18(14):2002-13.&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan, Republic of China. tcchang@po.iams.sinica.edu.tw</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tsai, Y. L.</style></author><author><style face="normal" font="default" size="100%">Wang, Z. F.</style></author><author><style face="normal" font="default" size="100%">Chen, W. W.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Emulsified BMVC derivative induced filtration for G-quadruplex DNA structural separation</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic Acids Res</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nucleic acids research</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*G-Quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbazoles/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA/*chemistry/*isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Emulsions</style></keyword><keyword><style  face="normal" font="default" size="100%">Filtration/*methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescent Dyes/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrocarbons, Brominated/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Microscopy, Fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">Potassium/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Pyridinium Compounds/*chemistry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Sep 1</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/21715373</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">17</style></number><edition><style face="normal" font="default" size="100%">2011/07/01</style></edition><volume><style face="normal" font="default" size="100%">39</style></volume><pages><style face="normal" font="default" size="100%">e114</style></pages><isbn><style face="normal" font="default" size="100%">1362-4962 (Electronic)0305-1048 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A novel method based on emulsion/filtration is introduced for G-quadruplex DNA structural separation. We first synthesized a lipophilic analogue of BMVC, 3,6-Bis(1-methyl-4-vinylpyridinium)-9-(12'-bromododecyl) carbazole diiodide (BMVC-12C-Br), which can form an oil-in-water (o/w) phase emulsion. Due to the binding preferences of BMVC-12C-Br emulsion to some specific DNA structures, the large emulsion ( approximately 2 microm) bound DNA was separated from the small free DNA in the filtrate by a 0.22 microm pore size MCE membrane. This method is able to isolate the non-parallel G-quadruplexes from the parallel G-quadruplexes and the linear duplexes from both G-quadruplexes. In addition, this method allows us not only to determine the absence of the parallel G-quadruplexes of d(T(2)AG(3))(4) and the presence of the parallel G-quadruplexes of d(T(2)AG(3))(2) in K(+) solution, but also to verify structural conversion from antiparallel to parallel G-quadruplexes of d[AG(3)(T(2)AG(3))(3)] in K(+) solution under molecular PEG condition. Moreover, this emulsion can separate the non-parallel G-quadruplexes of d(G(3)CGCG(3)AGGAAG(5)CG(3)) monomer from the parallel G-quadruplexes of its dimer in K(+) solution. Together with NMR spectra, one can simplify the spectra for both the free DNA and the bound DNA to establish a spectrum-structure correlation for further structural analysis.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Research Support, Non-U.S. Gov't</style></work-type><accession-num><style face="normal" font="default" size="100%">21715373</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Tsai, Yu-LinWang, Zi-FuChen, Wei-WenChang, Ta-ChauEnglandNucleic Acids Res. 2011 Sep 1;39(17):e114. doi: 10.1093/nar/gkr499. Epub 2011 Jun 28.&lt;/p&gt;
</style></notes><custom2><style face="normal" font="default" size="100%">3177220</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Chemistry, National Taiwan University, Taipei, Taiwan.</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Lin, C. T.</style></author><author><style face="normal" font="default" size="100%">Tseng, T. Y.</style></author><author><style face="normal" font="default" size="100%">Wang, Z. F.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Structural Conversion of Intramolecular and Intermolecular G-Quadruplexes of bcl2mid: The Effect of Potassium Concentration and Ion Exchange</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Physical Chemistry B</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Phys Chem B</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">arrangements</style></keyword><keyword><style  face="normal" font="default" size="100%">c-myc promoter</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA g-quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">human genome</style></keyword><keyword><style  face="normal" font="default" size="100%">human telomeric sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">k+ solution</style></keyword><keyword><style  face="normal" font="default" size="100%">molecule</style></keyword><keyword><style  face="normal" font="default" size="100%">region</style></keyword><keyword><style  face="normal" font="default" size="100%">STABILITY</style></keyword><keyword><style  face="normal" font="default" size="100%">transcription</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Mar 17</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000288113300026</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">10</style></number><volume><style face="normal" font="default" size="100%">115</style></volume><pages><style face="normal" font="default" size="100%">2360-2370</style></pages><isbn><style face="normal" font="default" size="100%">1520-6106</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The gel assay, circular dichroism, and differential scanning calorimetry results all demonstrate that a major monomer component of bcl2mid exists at low [K(+)] and an additional dimer component appears at high [K(+)]. This implies that bcl2mid is a good candidate for elucidating the mechanisms of structural conversion between different G-quadruplexes. We further discovered that the conversion between the monomer and dimer forms of bcl2mid does not occur at room temperature but is detected when heated above the melting point. In addition, the use of the lithium cation to keep the same ionic strength in a K(+) solution favors the formation of the bcl2mid dimer. We also found that the bcl2mid dimer is more stable than the monomer. However, after the bcl2mid monomer is formed in a K(+) solution, there is no appreciable structural conversion from the monomer to the dimer detected with addition of Li(+) at room temperature. Furthermore, the spectral changes of bcl2mid when transitioning from sodium form to potassium form take place upon K(+) titration. The absence of the dimer form for bcl2mid after the direct addition of 150 mM [K(+)] at room temperature suggests that the spectral changes are not due to rapid unfolding and refolding. In addition, this work reveals the conditions that would be useful for NMR studies of G-quadruplexes.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000288113300026</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;731NFTimes Cited:6Cited References Count:50&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 10617, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 10617, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 10617, TaiwanNatl Yang Ming Univ, Inst Biophoton, Taipei 11221, TaiwanNatl Taiwan Univ, Dept Chem, Taipei 10617, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chu, J. F.</style></author><author><style face="normal" font="default" size="100%">Wang, Z. F.</style></author><author><style face="normal" font="default" size="100%">Tseng, T. Y.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A Novel Method for Screening G-quadruplex Stabilizers to Human Telomeres</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of the Chinese Chemical Society</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Chin Chem Soc-Taip</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bmvc derivatives</style></keyword><keyword><style  face="normal" font="default" size="100%">cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">cells</style></keyword><keyword><style  face="normal" font="default" size="100%">conformational switch</style></keyword><keyword><style  face="normal" font="default" size="100%">copper induced unfolding</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Drug Design</style></keyword><keyword><style  face="normal" font="default" size="100%">g-quadruplex</style></keyword><keyword><style  face="normal" font="default" size="100%">inhibition</style></keyword><keyword><style  face="normal" font="default" size="100%">molecule</style></keyword><keyword><style  face="normal" font="default" size="100%">stabilizer</style></keyword><keyword><style  face="normal" font="default" size="100%">target</style></keyword><keyword><style  face="normal" font="default" size="100%">terminal transferase</style></keyword><keyword><style  face="normal" font="default" size="100%">yeast telomere</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jun</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000293668100005</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">3</style></number><volume><style face="normal" font="default" size="100%">58</style></volume><pages><style face="normal" font="default" size="100%">296-300</style></pages><isbn><style face="normal" font="default" size="100%">0009-4536</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;We present a simple method based on the Cu(2+) induced unfolding of G-quadruplex (G4) of human telomere sequence d[AG(3)(T(2)AG(3))(3)] to screen a number of 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC) analogues for better G4 stabilizers. Using circular dichroism (CD), the screening results suggest that the tri-cations of 9-substituted BMVC derivatives are better G4 stabilizers than the bi-cations of BMVC. In addition, 3,6-bis(1-methyl-4-vinylpyrazinium)carbazole diiodide (BMVC4) is likely a better core molecule than BM VC for G4 stabilizers.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000293668100005</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;804QCTimes Cited:0Cited References Count:37&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCAcad Sinica, Inst Atom &amp; Mol Sci, Taipei, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei, TaiwanNatl Taiwan Normal Univ, Dept Chem, Taipei, TaiwanNatl Taiwan Univ, Dept Chem, Taipei 10764, TaiwanNatl Yang Ming Univ, Inst Biophoton, Taipei 112, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chien, C. H.</style></author><author><style face="normal" font="default" size="100%">Chen, W. W.</style></author><author><style face="normal" font="default" size="100%">Wu, J. T.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Label-free imaging of Drosophila in vivo by coherent anti-Stokes Raman scattering and two-photon excitation autofluorescence microscopy</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Biomedical Optics</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Biomed Opt</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">caenorhabditis-elegans</style></keyword><keyword><style  face="normal" font="default" size="100%">cars microscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">coherent anti-stokes raman scattering microscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">drosophila</style></keyword><keyword><style  face="normal" font="default" size="100%">ENERGY</style></keyword><keyword><style  face="normal" font="default" size="100%">fat body</style></keyword><keyword><style  face="normal" font="default" size="100%">flies</style></keyword><keyword><style  face="normal" font="default" size="100%">in vivo</style></keyword><keyword><style  face="normal" font="default" size="100%">kynurenine</style></keyword><keyword><style  face="normal" font="default" size="100%">label-free imaging</style></keyword><keyword><style  face="normal" font="default" size="100%">melanogaster</style></keyword><keyword><style  face="normal" font="default" size="100%">metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">quantitative-analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">tissues</style></keyword><keyword><style  face="normal" font="default" size="100%">two-photon excitation autofluorescence microscopy</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000287636800030</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">1</style></number><volume><style face="normal" font="default" size="100%">16</style></volume><isbn><style face="normal" font="default" size="100%">1083-3668</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Drosophila is one of the most valuable model organisms for studying genetics and developmental biology. The fat body in Drosophila, which is analogous to the liver and adipose tissue in human, stores lipids that act as an energy source during its development. At the early stages of metamorphosis, the fat body remodeling occurs involving the dissociation of the fat body into individual fat cells. Here we introduce a combination of coherent anti-Stokes Raman scattering (CARS) and two-photon excitation autofluorescence (TPE-F) microscopy to achieve label-free imaging of Drosophila in vivo at larval and pupal stages. The strong CARS signal from lipids allows direct imaging of the larval fat body and pupal fat cells. In addition, the use of TPE-F microscopy allows the observation of other internal organs in the larva and autofluorescent globules in fat cells. During the dissociation of the fat body, the findings of the degradation of lipid droplets and an increase in autofluorescent globules indicate the consumption of lipids and the recruitment of proteins in fat cells. Through in vivo imaging and direct monitoring, CARS microscopy may help elucidate how metamorphosis is regulated and study the lipid metabolism in Drosophila. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3528642]&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000287636800030</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;725HWTimes Cited:7Cited References Count:36&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanNatl Yang Ming Univ, Inst Biophoton, Taipei 112, TaiwanNatl Taiwan Univ, Inst Mol Med, Coll Med, Taipei 100, TaiwanNatl Taiwan Univ Hosp, Dept Med Res, Taipei 100, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chu, J. F.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Li, H. W.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Single-Molecule TPM Studies on the Conversion of Human Telomeric DNA</style></title><secondary-title><style face="normal" font="default" size="100%">Biophysical Journal</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Biophys J</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BINDING</style></keyword><keyword><style  face="normal" font="default" size="100%">conformational switch</style></keyword><keyword><style  face="normal" font="default" size="100%">g-quadruplex structures</style></keyword><keyword><style  face="normal" font="default" size="100%">k+ solution</style></keyword><keyword><style  face="normal" font="default" size="100%">kinetics</style></keyword><keyword><style  face="normal" font="default" size="100%">potassium solution</style></keyword><keyword><style  face="normal" font="default" size="100%">quartet structures</style></keyword><keyword><style  face="normal" font="default" size="100%">sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">tethered particle motion</style></keyword><keyword><style  face="normal" font="default" size="100%">translocation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Apr 21</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000276939800028</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">8</style></number><volume><style face="normal" font="default" size="100%">98</style></volume><pages><style face="normal" font="default" size="100%">1608-1616</style></pages><isbn><style face="normal" font="default" size="100%">0006-3495</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Human telomere contains guanine-rich (G-rich) tandem repeats of single-stranded DNA sequences at its 3' tail. The G-rich sequences can be folded into various secondary structures, termed G-quadruplexes (G4s), by Hoogsteen basepairing in the presence of monovalent cations (such as Na(+) and K(+)). We developed a single-molecule tethered particle motion (TPM) method to investigate the unfolding process of G4s in the human telomeric sequence AGGG(TTAGGG)3 in real time. The TPM method monitors the DNA tether length change caused by formation of the G4, thus allowing the unfolding process and structural conversion to be monitored at the single-molecule level. In the presence of its antisense sequence, the folded G4 structure can be disrupted and converted to the unfolded conformation, with apparent unfolding time constants of 82 s and 3152 s. We also observed that the stability of the G4 is greatly affected by different monovalent cations. The folding equilibrium constant of G4 is strongly dependent on the salt concentration, ranging from 1.75 at 5 mM Na(+) to 3.40 at 15 mM Na(+). Earlier spectral studies of Na(+)- and K(+)-folded states suggested that the spectral conversion between these two different folded structures may go through a structurally unfolded intermediate state. However, our single-molecule TPM experiments did not detect any totally unfolded intermediate within our experimental resolution when sodium-folded G4 DNA molecules were titrated with high-concentration, excess potassium ions. This observation suggests that a totally unfolding pathway is likely not the major pathway for spectral conversion on the timescale of minutes, and that interconversion among folded states can be achieved by the loop rearrangement. This study also demonstrates that TPM experiments can be used to study conformational changes in single-stranded DNA molecules.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000276939800028</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;586UTTimes Cited:5Cited References Count:42&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Li, HwNatl Taiwan Univ, Dept Chem, Taipei 10764, TaiwanNatl Taiwan Univ, Dept Chem, Taipei 10764, TaiwanNatl Taiwan Univ, Dept Chem, Taipei 10764, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei, TaiwanNatl Taiwan Normal Univ, Dept Chem, Taipei, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Detection of G-quadruplexes in cells and investigation of G-quadruplex structure of d(T2AG3)4 in K+ solution by a carbazole derivative: BMVC</style></title><secondary-title><style face="normal" font="default" size="100%">Methods Mol Biol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*G-Quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">Adenocarcinoma/genetics/pathology</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbazoles/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Computer Simulation</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA/*chemistry/ultrastructure</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescent Dyes</style></keyword><keyword><style  face="normal" font="default" size="100%">Guanine/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Lung Neoplasms/genetics/pathology</style></keyword><keyword><style  face="normal" font="default" size="100%">Potassium/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Pyridinium Compounds/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Solutions</style></keyword><keyword><style  face="normal" font="default" size="100%">Telomere/*chemistry/ultrastructure</style></keyword><keyword><style  face="normal" font="default" size="100%">Tumor Cells, Cultured</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/20012423</style></url></web-urls></urls><edition><style face="normal" font="default" size="100%">2009/12/17</style></edition><volume><style face="normal" font="default" size="100%">608</style></volume><pages><style face="normal" font="default" size="100%">183-206</style></pages><isbn><style face="normal" font="default" size="100%">1940-6029 (Electronic)1064-3745 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Verification of the existence of quadruplex structure in native human telomeres and determination of the major structure of d(T(2)AG(3))(4) (H24) in K(+) solution are the major questions regarding the structure of human telomeres. We have synthesized a fluorescent probe of 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC) that has a very high binding affinity for G-quadruplex H24. BMVC stabilizes quadruplex structures and acts as a sensitive probe to the local environment. Although the circular dichroism patterns of H24 are different in Na(+) and K(+) solutions, similar binding behaviors of BMVC to H24 in these solutions led us to suggest that the major G-quadruplex structure of H24 in K(+) solution is very likely similar to that in Na(+) solution. Of particular interest is the fluorescent band detected at -575 nm in quadruplex H24 and at -545 nm in duplex DNA. In addition, the intensity of BMVC fluorescence increases by two orders of magnitudes upon interaction with either duplex or G-quadruplex DNA. BMVC has a greater binding preference for G-quadruplex H24 than for duplex DNA. Analyzing the BMVC fluorescence at the ends of metaphase chromosomes and other regions of chromosomes allowed us to verify the presence of G-quadruplex structure in human telomeres for the first time. Using fluorescence lifetime imaging microscopy, the longer decay time of BMVC in G-quadruplex H24 than in duplex DNA allowed us to map the G-quadruplex structure in human metaphase chromosomes.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Research Support, Non-U.S. Gov't</style></work-type><accession-num><style face="normal" font="default" size="100%">20012423</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Chang, Ta-ChauChang, Cheng-ChungClifton, N.J.Methods Mol Biol. 2010;608:183-206. doi: 10.1007/978-1-59745-363-9_12.&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Institute of Atomic and Molecular Sciences, and Genomic Research Center, Academia Sinica, Taipei, Taiwan, Republic of China.</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Liao, L. J.</style></author><author><style face="normal" font="default" size="100%">Kang, C. C.</style></author><author><style face="normal" font="default" size="100%">Jan, I. S.</style></author><author><style face="normal" font="default" size="100%">Chen, H. C.</style></author><author><style face="normal" font="default" size="100%">Wang, C. L.</style></author><author><style face="normal" font="default" size="100%">Lou, P. J.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Improved diagnostic accuracy of malignant neck lumps by a simple BMVC staining assay</style></title><secondary-title><style face="normal" font="default" size="100%">Analyst</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Analyst</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">biopsy</style></keyword><keyword><style  face="normal" font="default" size="100%">cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">limitations</style></keyword><keyword><style  face="normal" font="default" size="100%">needle-aspiration-cytology</style></keyword><keyword><style  face="normal" font="default" size="100%">tool</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000264482300010</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">4</style></number><volume><style face="normal" font="default" size="100%">134</style></volume><pages><style face="normal" font="default" size="100%">708-711</style></pages><isbn><style face="normal" font="default" size="100%">0003-2654</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A handheld device based on fluorescence of 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC) staining was established for the rapid, point-of-care screening of cancer cells (see Chang and co-workers, Analyst, 2007, 132, 745). Offering instant screening of cancer at low cost, here we apply this simple assay in clinical tests on fine needle aspirates of neck masses from 114 outpatients (115 specimens). The diagnostic accuracy of this simple method alone is ca. 80% (80/99). The combination of the BMVC test and the fine needle aspiration (FNA) cytology reduced the non-diagnosis from 17 cases in FNA cytology to 6 cases in the combined method. Moreover, an algorithm is proposed to improve the diagnostic accuracy of malignant neck lumps up to nearly 100%.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000264482300010</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;423FNTimes Cited:1Cited References Count:12&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Lou, PJNatl Taiwan Univ Hosp, Dept Otolaryngol, Taipei, TaiwanNatl Taiwan Univ Hosp, Dept Otolaryngol, Taipei, TaiwanNatl Taiwan Univ Hosp, Dept Otolaryngol, Taipei, TaiwanNatl Taiwan Univ, Coll Med, Taipei 10764, TaiwanFar Eastern Mem Hosp, Dept Otolaryngol, Taipei, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanNatl Tsing Hua Univ, Taiwan Int Grad Program, Hsinchu, TaiwanNatl Tsing Hua Univ, Dept Chem, Hsinchu, TaiwanNatl Taiwan Univ, Coll Med, Taipei 10764, TaiwanNatl Taiwan Univ Hosp, Dept Lab Med, Taipei, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chen, C. T.</style></author><author><style face="normal" font="default" size="100%">Cho, C. C.</style></author><author><style face="normal" font="default" size="100%">Lin, Y. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A dual selective antitumor agent and fluorescence probe: the binary BMVC-porphyrin photosensitizer</style></title><secondary-title><style face="normal" font="default" size="100%">Chemmedchem</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Chemmedchem</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">cell death markers</style></keyword><keyword><style  face="normal" font="default" size="100%">cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">fret</style></keyword><keyword><style  face="normal" font="default" size="100%">lifetime</style></keyword><keyword><style  face="normal" font="default" size="100%">localization</style></keyword><keyword><style  face="normal" font="default" size="100%">photodynamic therapy</style></keyword><keyword><style  face="normal" font="default" size="100%">photoinduced translocation</style></keyword><keyword><style  face="normal" font="default" size="100%">series</style></keyword><keyword><style  face="normal" font="default" size="100%">singlet oxygen</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000256183300003</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">5</style></number><volume><style face="normal" font="default" size="100%">3</style></volume><pages><style face="normal" font="default" size="100%">725-728</style></pages><isbn><style face="normal" font="default" size="100%">1860-7179</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">n/a</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000256183300003</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;305MUTimes Cited:3Cited References Count:22&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Kang, CCAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanNatl Tsing Hua Univ, Taiwan Int Grad Program, Hsinchu, TaiwanNatl Tsing Hua Univ, Dept Chem, Hsinchu, TaiwanAcad Sinica, Taipei, TaiwanNatl Taiwan Univ, Coll Med, Ctr Optoelect Biomed, Taipei 10764, TaiwanNatl Taiwan Univ, Coll Life Sci, Inst Microbiol &amp; Biochem, Taipei 10764, TaiwanNatl Chung Hsing Univ, Dept Chem, Taichung 40227, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Huang, F. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Lou, P. J.</style></author><author><style face="normal" font="default" size="100%">Kuo, I. C.</style></author><author><style face="normal" font="default" size="100%">Chien, C. W.</style></author><author><style face="normal" font="default" size="100%">Chen, C. T.</style></author><author><style face="normal" font="default" size="100%">Shieh, F. Y.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Lin, J. J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">G-quadruplex stabilizer 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide induces accelerated senescence and inhibits tumorigenic properties in cancer cells</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular Cancer Research</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Mol Cancer Res</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA structures</style></keyword><keyword><style  face="normal" font="default" size="100%">Drug Design</style></keyword><keyword><style  face="normal" font="default" size="100%">human genome</style></keyword><keyword><style  face="normal" font="default" size="100%">human telomerase</style></keyword><keyword><style  face="normal" font="default" size="100%">Ligands</style></keyword><keyword><style  face="normal" font="default" size="100%">proliferation</style></keyword><keyword><style  face="normal" font="default" size="100%">promoter region</style></keyword><keyword><style  face="normal" font="default" size="100%">small-molecule</style></keyword><keyword><style  face="normal" font="default" size="100%">target</style></keyword><keyword><style  face="normal" font="default" size="100%">transcription</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jun</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000257098200008</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">6</style></number><volume><style face="normal" font="default" size="100%">6</style></volume><pages><style face="normal" font="default" size="100%">955-964</style></pages><isbn><style face="normal" font="default" size="100%">1541-7786</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Carbazole derivatives that stabilized G-quadruplex DNA structure formed by human telomeric sequence have been designed and synthesized. Among them, 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC) showed an increase in G-quadruplex melting temperature by 13 degrees C and has a potent inhibitory effect on telomerase activity. Treatment of H1299 cancer cells with 0.5 mu mol/L BMVC did not cause acute toxicity and affect DNA replication; however, the BMVC-treated cells ceased to divide after a lag period. Hallmarks of senescence, including morphologic changes, detection of senescence-associated beta-galactosidase activity, and decreased bromodeoxyuridine incorporation, were detected in BMVC-treated cancer cells. The BMVC-induced senescence phenotype is accompanied by progressive telomere shortening and detection of the DNA damage foci, indicating that BMVC caused telomere uncapping after long-term treatments. Unlike other telomerase inhibitors, the BMVC-treated cancer cells showed a fast telomere shortening rate and a lag period of growth before entering senescence. Interestingly, BMVC also suppressed the tumor-related properties of cancer cells, including cell migration, colony-forming ability, and anchorage-independent growth, indicating that the cellular effects of BMVC were not limited to telomeres. Consistent with the observations from cellular experiments, the tumorigenic potential of cancer cells was also reduced in mouse xenografts after BMVC treatments. Thus, BMVC repressed tumor progression through both telomere-dependent and telomere-independent pathways.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000257098200008</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;318NHTimes Cited:14Cited References Count:50&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Lin, JJNatl Yang Ming Univ, Inst Biopharmaceut Sci, Taipei 11221, TaiwanNatl Yang Ming Univ, Inst Biopharmaceut Sci, Taipei 11221, TaiwanNatl Yang Ming Univ, Inst Biopharmaceut Sci, Taipei 11221, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei, TaiwanNatl Taiwan Univ Hosp, Dept Otolaryngol, Taipei, TaiwanNatl Taiwan Univ, Coll Med, Ctr Optoelect Biomed, Taipei 10764, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kao, W. C.</style></author><author><style face="normal" font="default" size="100%">Wang, V. C. C.</style></author><author><style face="normal" font="default" size="100%">Huang, Y. C.</style></author><author><style face="normal" font="default" size="100%">Yu, S. S. F.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Chan, S. I.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation, purification and characterization of hemerythrin from Methylococcus capsulatus (Bath)</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Inorganic Biochemistry</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Inorg Biochem</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bacterial chemotaxis protein</style></keyword><keyword><style  face="normal" font="default" size="100%">domain</style></keyword><keyword><style  face="normal" font="default" size="100%">electron paramagnetic resonance</style></keyword><keyword><style  face="normal" font="default" size="100%">hemerythrin</style></keyword><keyword><style  face="normal" font="default" size="100%">methane monooxygenase</style></keyword><keyword><style  face="normal" font="default" size="100%">methylococcus capsulatus (bath)</style></keyword><keyword><style  face="normal" font="default" size="100%">multiple sequence alignment</style></keyword><keyword><style  face="normal" font="default" size="100%">particulate methane monooxygenase</style></keyword><keyword><style  face="normal" font="default" size="100%">protein purification</style></keyword><keyword><style  face="normal" font="default" size="100%">resonance raman spectroscopy</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Aug</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000258012000007</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">8</style></number><volume><style face="normal" font="default" size="100%">102</style></volume><pages><style face="normal" font="default" size="100%">1607-1614</style></pages><isbn><style face="normal" font="default" size="100%">0162-0134</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Earlier work from our laboratory has indicated that a hemerythrin-like protein was over-produced together with the particulate methane monooxygenase (pMMO) when Methylococcus capsulatus (Bath) was grown under high copper concentrations. A homologue of hemerythrin had not previously been found in any prokaryote. To confirm its identity as a hemerythrin, we have isolated and purified this protein by ion-exchange, gel-filtration and hydrophobic interaction chromatography, and characterized it by mass spectrometry, UV-visible, CD, EPR and resonance Raman spectroscopy. On the basis of biophysical and multiple sequence alignment analysis, the protein isolated from M. capsulatus (Bath) is in accord with hemerythrins previously reported from higher organisms. Determination of the Fe content in conjunction with molecular-weight estimation and mass analysis indicates that the native hemerythrin in M. capsulatus (Bath) is a monomer with molecular mass 14.8 kDa, in contrast to hemerythrins from other eukaryotic organisms, where they typically exist as a tetramer or higher oligomers. (c) 2008 Elsevier Inc. All rights reserved.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000258012000007</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;331KVTimes Cited:13Cited References Count:23&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chan, SIAcad Sinica, Inst Chem, Taipei 115, TaiwanAcad Sinica, Inst Chem, Taipei 115, TaiwanAcad Sinica, Inst Chem, Taipei 115, TaiwanNatl Taiwan Univ, Dept Chem, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Yang, D. Y.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Sheu, S. Y.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Interaction between human telomere and a carbazole derivative: A molecular dynamics simulation of a quadruplex stabilizer and telomerase inhibitor</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Physical Chemistry A</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Phys Chem A</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bmvc</style></keyword><keyword><style  face="normal" font="default" size="100%">cancer-cells</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA structures</style></keyword><keyword><style  face="normal" font="default" size="100%">Drug Design</style></keyword><keyword><style  face="normal" font="default" size="100%">fluorescence probe</style></keyword><keyword><style  face="normal" font="default" size="100%">human fibroblasts</style></keyword><keyword><style  face="normal" font="default" size="100%">potent</style></keyword><keyword><style  face="normal" font="default" size="100%">target</style></keyword><keyword><style  face="normal" font="default" size="100%">terminal transferase</style></keyword><keyword><style  face="normal" font="default" size="100%">tumor-cells</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Sep 27</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000249655600006</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">38</style></number><volume><style face="normal" font="default" size="100%">111</style></volume><pages><style face="normal" font="default" size="100%">9224-9232</style></pages><isbn><style face="normal" font="default" size="100%">1089-5639</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The mechanism of inhibition of telomerase by drugs is a key factor in an understanding of guanine-quadruplex complex stabilization during human cancer. This study describes a simulated annealing docking and molecular dynamics simulation to investigate a synthesized potent inhibitor, 3,6-bis(1-methyl-4-vinylpyridinium iodine) carbazole (BMVC), which stabilizes the quadruplex structure of the human telomeric DNA sequence d[AG(3)(T(2)AG(3))(3)] and inhibits telomerase activity. The compound was predicted to selectively interact with the quadruplex structure. During our simulation, the binding affinities were calculated and used to predict the best drug-binding sites as well as enhanced selectivity compared with other compounds. Our studies suggest that the simulation results quite coincide with the experimental results. In addition, molecular modeling shows that a 2:1 binding model involving the external binding of BMVC to both ends of the G-quartet of d[AG(3)(T(2)AG(3))(3)] is the most stable binding mode and this agrees with the absorbance titration results that show two binding sites. Of particular interest is that one pyridinium ring and carbazole moiety of the BMVC can stack well at the end of G-quartet. This implies that BMVC is a good human quadruplex stabilizer and also a good telomerase inhibitor.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000249655600006</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;213GTTimes Cited:17Cited References Count:54&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Sheu, SYNatl Yang Ming Univ, Inst Bioinformat, Struct Biol Program, Dept Life Sci, Tokyo 112, JapanNatl Yang Ming Univ, Inst Bioinformat, Struct Biol Program, Dept Life Sci, Tokyo 112, JapanNatl Yang Ming Univ, Inst Bioinformat, Struct Biol Program, Dept Life Sci, Tokyo 112, JapanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tsai, Y. L.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Kang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effect of different electronic properties on 9-aryl-substituted BMVC derivatives for new fluorescence probes</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Luminescence</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Lumin</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">florescence probe</style></keyword><keyword><style  face="normal" font="default" size="100%">human telomeres</style></keyword><keyword><style  face="normal" font="default" size="100%">intramolecular charge transfer</style></keyword><keyword><style  face="normal" font="default" size="100%">laser-dyes</style></keyword><keyword><style  face="normal" font="default" size="100%">molecular rotor</style></keyword><keyword><style  face="normal" font="default" size="100%">molecular rotors</style></keyword><keyword><style  face="normal" font="default" size="100%">photophysical properties</style></keyword><keyword><style  face="normal" font="default" size="100%">quadruplex structure</style></keyword><keyword><style  face="normal" font="default" size="100%">sensitivity</style></keyword><keyword><style  face="normal" font="default" size="100%">SYSTEMS</style></keyword><keyword><style  face="normal" font="default" size="100%">torsional relaxation</style></keyword><keyword><style  face="normal" font="default" size="100%">viscosity dependence</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Nov</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000247332000009</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">1</style></number><volume><style face="normal" font="default" size="100%">127</style></volume><pages><style face="normal" font="default" size="100%">41-47</style></pages><isbn><style face="normal" font="default" size="100%">0022-2313</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;We have previously illustrated that the electron donor of carbazole moiety and the electron acceptor of methyl pyridinium cation in 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC) molecule could form an intramolecular charge-transfer state. The intramolecular twist of the vinyl group in bridging the donor and acceptor plays an important role in the BMVC fluorescence. Here, we have synthesized three 9-aryl-substituted BMVC derivatives with different electronic properties for the design of the second generation of fluorescence probes. The steady-state solvatochromic studies show no appreciable change to the charge transfer of BMVC by substituting an anisole electron-donating group at 9-position of BMVC. However, substituting a 9-nitrobenzyl electron-withdrawing group in BMVC could restrict the charge transfer in the excited state. Moreover, the increase of the fluorescence yields of 9-anisole BMVC and 9-phenyl BMVC upon interaction with DNA is even higher than that in glycerol, while the fluorescence yield of 9-nitrobenzyl BMVC upon interaction with DNA is much lower than that in glycerol. Although 9-nitrobenzyl BMVC is a good G-quadruplex stabilizer, substituting an electron-withdrawing group at 9-position of BMVC is not recommended for the design of fluorescence probes. On the other hand, colocalization between 9-phenyl BMVC and MitoTracker Red in the merged image of cells indicates that the 9-phenyl BMVC is a potential fluorescent mitochondrial probe. (C) 2007 Elsevier B.V. All rights reserved.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000247332000009</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;180AKTimes Cited:8Cited References Count:22&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCNatl Chung Hsin Univ, Dept Chem, Hsinchu, TaiwanNatl Chung Hsin Univ, Dept Chem, Hsinchu, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanNatl Tsing Hua Univ, Acad Sinica, Dept Chem, Taiwan Int Grad Program, Tsinghua, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chien, C. W.</style></author><author><style face="normal" font="default" size="100%">Lin, Y. H.</style></author><author><style face="normal" font="default" size="100%">Kang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Investigation of spectral conversion of d(TTAGGG)(4) and d(TTAGGG)(13) upon potassium titration by a G-quadruplex recognizer BMVC molecule</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic Acids Research</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nucleic Acids Res</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antiparallel</style></keyword><keyword><style  face="normal" font="default" size="100%">BINDING</style></keyword><keyword><style  face="normal" font="default" size="100%">circular-dichroism</style></keyword><keyword><style  face="normal" font="default" size="100%">crystal-structure</style></keyword><keyword><style  face="normal" font="default" size="100%">d(g(4)t(4)g(4))</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">human telomere</style></keyword><keyword><style  face="normal" font="default" size="100%">parallel</style></keyword><keyword><style  face="normal" font="default" size="100%">sequence forms</style></keyword><keyword><style  face="normal" font="default" size="100%">tetraplex</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000247350800004</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">9</style></number><volume><style face="normal" font="default" size="100%">35</style></volume><pages><style face="normal" font="default" size="100%">2846-2860</style></pages><isbn><style face="normal" font="default" size="100%">0305-1048</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;We have introduced a G- quadruplex- binding ligand, 3,6- bis( 1- methyl- 4- vinylpyridinium) carbazole diiodide ( BMVC), to verify the major structure of d( T(2)AG(3))(4) ( H24) in potassium solution and examine the structural conversion of H24 in sodium solution upon potassium titration. The studies of circular dichroism, induced circular dichroism, spectral titration and gel competition have allowed us to determine the binding mode and binding ratio of BMVC to the H24 in solution and eliminate the parallel form as the major G- quadruplex structure. Although the mixed- type form could not be eliminated as a main component, the basket and chair forms are more likely the main components of H24 in potassium solution. In addition, the circular dichroism spectra and the job plots reveal that a longer telomeric sequence d( T(2)AG(3))(13) ( H78) could form two units of G4 structure both in sodium or potassium solutions. Of particular interest is that no appreciable change on the induced circular dichroism spectra of BMVC is found during the change of the circular dichroism patterns of H24 upon potassium titration. Considering similar spectral conversion detected for H24 and a long sequence H78 together with the G4 structure stabilized by BMVC, it is therefore unlikely that the rapid spectral conversion of H24 and H78 is due to structural change between different types of the G4 structures. With reference to the circular dichroism spectra of d( GAA)(7) and d( GAAA)(5), we suggest that the spectral conversion of H24 upon potassium titration is attributed to fast ion exchange resulting in different loop base interaction and various hydrogen bonding effects.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000247350800004</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;180GKTimes Cited:43Cited References Count:45&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanNatl Chung Hsing Univ, Dept Chem, Taichung 40227, TaiwanNatl Yang Ming Univ, Inst Biophoton Engn, Taipei 11221, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Liao, L. J.</style></author><author><style face="normal" font="default" size="100%">Lou, P. J.</style></author><author><style face="normal" font="default" size="100%">Xie, W.</style></author><author><style face="normal" font="default" size="100%">Yeung, E. S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A handheld device for potential point-of-care screening of cancer</style></title><secondary-title><style face="normal" font="default" size="100%">Analyst</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Analyst</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bmvc</style></keyword><keyword><style  face="normal" font="default" size="100%">cells</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">flow-cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">human telomeres</style></keyword><keyword><style  face="normal" font="default" size="100%">quadruplex structure</style></keyword><keyword><style  face="normal" font="default" size="100%">SPECTROSCOPY</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000248229700014</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">8</style></number><volume><style face="normal" font="default" size="100%">132</style></volume><pages><style face="normal" font="default" size="100%">745-749</style></pages><isbn><style face="normal" font="default" size="100%">0003-2654</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A simple handheld device based on the fluorescence analysis of 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide ( BMVC) stained cells was established for routine screening and potentially for early detection of cancer cells at extremely low cost. Flow cytometry assay further supported the utility of this simple device, where a preliminary study of tissue biopsy showed highly encouraging results.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000248229700014</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;192UUTimes Cited:14Cited References Count:15&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCNatl Chung Hsing Univ, Dept Chem, 250 Kuokuang Rd, Taichung, TaiwanNatl Chung Hsing Univ, Dept Chem, 250 Kuokuang Rd, Taichung, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanNatl Tsing Hua Univ, Dept Chem, Hsinchu 30013, TaiwanAcad Sinica, Taipei 115, TaiwanNatl Taiwan Univ Hosp, Dept Otorhinolaryngol, Taipei, TaiwanNatl Taiwan Univ, Coll Med, Taipei, TaiwanUS DOE, Ames Lab, Ames, IA 50011 USAIowa State Univ, Dept Chem, Ames, IA 50011 USA</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chien, C. W.</style></author><author><style face="normal" font="default" size="100%">Lin, Y. H.</style></author><author><style face="normal" font="default" size="100%">Kang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Investigation of spectral conversion of d(TTAGGG)4 and d(TTAGGG)13 upon potassium titration by a G-quadruplex recognizer BMVC molecule</style></title><secondary-title><style face="normal" font="default" size="100%">Nucleic Acids Res</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nucleic acids research</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Binding Sites</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbazoles/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Circular Dichroism</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis, Polyacrylamide Gel</style></keyword><keyword><style  face="normal" font="default" size="100%">G-Quadruplexes</style></keyword><keyword><style  face="normal" font="default" size="100%">Guanine/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Nucleic Acid Conformation</style></keyword><keyword><style  face="normal" font="default" size="100%">Potassium/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Pyridinium Compounds/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Sodium/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Spectrophotometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Telomere/*chemistry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17430965</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">9</style></number><edition><style face="normal" font="default" size="100%">2007/04/14</style></edition><volume><style face="normal" font="default" size="100%">35</style></volume><pages><style face="normal" font="default" size="100%">2846-60</style></pages><isbn><style face="normal" font="default" size="100%">1362-4962 (Electronic)0305-1048 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;We have introduced a G-quadruplex-binding ligand, 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC), to verify the major structure of d(T2AG3)4 (H24) in potassium solution and examine the structural conversion of H24 in sodium solution upon potassium titration. The studies of circular dichroism, induced circular dichroism, spectral titration and gel competition have allowed us to determine the binding mode and binding ratio of BMVC to the H24 in solution and eliminate the parallel form as the major G-quadruplex structure. Although the mixed-type form could not be eliminated as a main component, the basket and chair forms are more likely the main components of H24 in potassium solution. In addition, the circular dichroism spectra and the job plots reveal that a longer telomeric sequence d(T2AG3)13 (H78) could form two units of G4 structure both in sodium or potassium solutions. Of particular interest is that no appreciable change on the induced circular dichroism spectra of BMVC is found during the change of the circular dichroism patterns of H24 upon potassium titration. Considering similar spectral conversion detected for H24 and a long sequence H78 together with the G4 structure stabilized by BMVC, it is therefore unlikely that the rapid spectral conversion of H24 and H78 is due to structural change between different types of the G4 structures. With reference to the circular dichroism spectra of d(GAA)7 and d(GAAA)5, we suggest that the spectral conversion of H24 upon potassium titration is attributed to fast ion exchange resulting in different loop base interaction and various hydrogen bonding effects.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Research Support, Non-U.S. Gov't</style></work-type><accession-num><style face="normal" font="default" size="100%">17430965</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Chang, Cheng-ChungChien, Chih-WeiLin, Yi-HsuehKang, Chi-ChihChang, Ta-ChauEnglandNucleic Acids Res. 2007;35(9):2846-60. Epub 2007 Apr 11.&lt;/p&gt;
</style></notes><custom2><style face="normal" font="default" size="100%">1888807</style></custom2><auth-address><style face="normal" font="default" size="100%">Institute of Atomic and Molecular Sciences, Academia Sinica, PO Box 23-166, Taipei, 106, Taiwan, Republic of China. tcchang@po.iams.sinica.edu.tw</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chu, J. F.</style></author><author><style face="normal" font="default" size="100%">Kao, F. J.</style></author><author><style face="normal" font="default" size="100%">Lou, P. J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Detection of quadruplex DNA structures in human telomeres by using a fluorescence probe BMVC molecule</style></title><secondary-title><style face="normal" font="default" size="100%">Abstracts of Papers of the American Chemical Society</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Abstr Pap Am Chem S</style></alt-title></titles><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Sep 10</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000207781600708</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">232</style></volume><pages><style face="normal" font="default" size="100%">805-805</style></pages><isbn><style face="normal" font="default" size="100%">0065-7727</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">n/a</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000207781600708</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;V15db109-BIOLTimes Cited:0Cited References Count:0&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Acad Sinica, Inst Atom &amp; Mol Sci, Taipei 10617, TaiwanNatl Yang Ming Univ, Inst Biophoton Engn, Taipei 11221, TaiwanNatl Taiwan Univ Hosp, Dept Otolaryngol, Taipei, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">G-quadruplex structure of d(TTAGGG)4 in potassium solution investigated by BMVC molecule</style></title><secondary-title><style face="normal" font="default" size="100%">Abstracts of Papers of the American Chemical Society</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Abstr Pap Am Chem S</style></alt-title></titles><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Sep 10</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000207781600706</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">232</style></volume><pages><style face="normal" font="default" size="100%">803-803</style></pages><isbn><style face="normal" font="default" size="100%">0065-7727</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">n/a</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000207781600706</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;V15db119-BIOLTimes Cited:0Cited References Count:0&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Acad Sinica, Inst Atom &amp; Mol Sci, Taipei 10617, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chu, J. F.</style></author><author><style face="normal" font="default" size="100%">Kuo, H. H.</style></author><author><style face="normal" font="default" size="100%">Kang, C. C.</style></author><author><style face="normal" font="default" size="100%">Lin, S. H.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Solvent effect on photophysical properties of a fluorescence probe: BMVC</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Luminescence</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Lumin</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bmvc</style></keyword><keyword><style  face="normal" font="default" size="100%">carbazole</style></keyword><keyword><style  face="normal" font="default" size="100%">chromophores</style></keyword><keyword><style  face="normal" font="default" size="100%">DYNAMICS</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">laser-dyes</style></keyword><keyword><style  face="normal" font="default" size="100%">photoluminescence</style></keyword><keyword><style  face="normal" font="default" size="100%">photophysical properties</style></keyword><keyword><style  face="normal" font="default" size="100%">quadruplex DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">relaxation</style></keyword><keyword><style  face="normal" font="default" size="100%">sensitivity</style></keyword><keyword><style  face="normal" font="default" size="100%">viscosity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jul-Oct</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000236925800019</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">119</style></volume><pages><style face="normal" font="default" size="100%">84-90</style></pages><isbn><style face="normal" font="default" size="100%">0022-2313</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Fluorescence studies of 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC) in glycerol/water mixtures allow us to elucidate the photophysical behavior of BMVC upon interaction with different DNA structures. The very weak fluorescence emission of BMVC in highly polar solvents of water is attributed to an increase in nonradiative decay due to the intramolecular twist of the vinyl group induced by charge transfer. Increasing the solvent viscosity and rigidity could lead to large changes in the barrier height and substantial effects on relaxation processes, and result in an enhancement of the fluorescence quantum yield. Similarly, different binding interactions of BMVC with various DNA could perturb the frictions of the reorientation of the vinyl group. We suggest that the intramolecular twist of the vinyl group of BMVC is mainly responsible for the distinct fluorescence emissions under different local environments. (c) 2006 Elsevier B.V. All rights reserved.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000236925800019</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;034JRTimes Cited:21Cited References Count:17&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanNatl Taiwan Normal Univ, Dept Chem, Taipei, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tsai, C. H.</style></author><author><style face="normal" font="default" size="100%">Chan, P. H.</style></author><author><style face="normal" font="default" size="100%">Lin, C. H.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Chia, C. T.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode</style></title><secondary-title><style face="normal" font="default" size="100%">Electrophoresis</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Electrophoresis</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">capillary-electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">ce</style></keyword><keyword><style  face="normal" font="default" size="100%">dynamic ph junction</style></keyword><keyword><style  face="normal" font="default" size="100%">fluorescence detection</style></keyword><keyword><style  face="normal" font="default" size="100%">identification</style></keyword><keyword><style  face="normal" font="default" size="100%">leucomalachite green</style></keyword><keyword><style  face="normal" font="default" size="100%">malachite green</style></keyword><keyword><style  face="normal" font="default" size="100%">mass-spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">performance liquid-chromatography</style></keyword><keyword><style  face="normal" font="default" size="100%">raman spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">SCATTERING</style></keyword><keyword><style  face="normal" font="default" size="100%">stacking</style></keyword><keyword><style  face="normal" font="default" size="100%">surface enhanced-resonance raman spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">sweeping-mekc</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Dec</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000242903000011</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">23</style></number><volume><style face="normal" font="default" size="100%">27</style></volume><pages><style face="normal" font="default" size="100%">4688-4693</style></pages><isbn><style face="normal" font="default" size="100%">0173-0835</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm(-1) (N-phi and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000242903000011</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;117WCTimes Cited:9Cited References Count:27&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Lin, CHNatl Taiwan Normal Univ, Dept Chem, 88 Sec 4,Tingchow Rd, Taipei, TaiwanNatl Taiwan Normal Univ, Dept Chem, 88 Sec 4,Tingchow Rd, Taipei, TaiwanNatl Taiwan Normal Univ, Dept Chem, Taipei, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei, TaiwanNatl Taiwan Normal Univ, Dept Phys, Taipei, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chu, J. F.</style></author><author><style face="normal" font="default" size="100%">Kao, F. J.</style></author><author><style face="normal" font="default" size="100%">Chiu, Y. C.</style></author><author><style face="normal" font="default" size="100%">Lou, P. J.</style></author><author><style face="normal" font="default" size="100%">Chen, H. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Verification of antiparallel G-quadruplex structure in human telomeres by using two-photon excitation fluorescence lifetime imaging microscopy of the 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide molecule</style></title><secondary-title><style face="normal" font="default" size="100%">Analytical Chemistry</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Anal Chem</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">c-myc promoter</style></keyword><keyword><style  face="normal" font="default" size="100%">carbazole</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Drug Design</style></keyword><keyword><style  face="normal" font="default" size="100%">DYNAMICS</style></keyword><keyword><style  face="normal" font="default" size="100%">region</style></keyword><keyword><style  face="normal" font="default" size="100%">target</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Apr 15</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000236948300046</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">8</style></number><volume><style face="normal" font="default" size="100%">78</style></volume><pages><style face="normal" font="default" size="100%">2810-2815</style></pages><isbn><style face="normal" font="default" size="100%">0003-2700</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Different G-quadruplex structures for the human telomeric sequence d(T(2)AG(3))(4) in vitro have been documented in the presence of sodium and potassium. Verification of the G-quadruplex structures in human telomeres in vivo is the main issue in establishing the biological function of the G-quadruplex structures in telomeres as well as the development of anticancer agents. Here we have applied two-photon excitation fluorescence lifetime imaging microscopy to measure the fluorescence lifetime of the BMVC molecule upon interaction with various DNA structures. The distinction in lifetime measured with submicrometer spatial resolution in two-photon excitation fluorescence lifetime imaging microscopy provides a powerful approach not only to verify the existence of the antiparallel G-quadruplex structure in human telomeres but also to map its localizations in metaphase chromosomes.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000236948300046</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;034RMTimes Cited:48Cited References Count:26&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanNatl Taiwan Normal Univ, Dept Chem, Taipei, TaiwanNatl Yang Ming Univ, Inst Biophoton Engn, Taipei 11221, TaiwanNatl Sun Yat Sen Univ, Inst Electroopt Engn, Kaohsiung 80424, TaiwanNatl Taiwan Univ Hosp, Dept Otolaryngol, Taipei, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hecht, C.</style></author><author><style face="normal" font="default" size="100%">Hermann, P.</style></author><author><style face="normal" font="default" size="100%">Friedrich, J.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Thionin in a cyclodextrin nanocavity: Measuring local compressibilities by pressure tuning hole burning spectroscopy</style></title><secondary-title><style face="normal" font="default" size="100%">Chemical Physics Letters</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Chem Phys Lett</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">beta-cyclodextrin</style></keyword><keyword><style  face="normal" font="default" size="100%">COMPLEXES</style></keyword><keyword><style  face="normal" font="default" size="100%">dye</style></keyword><keyword><style  face="normal" font="default" size="100%">globular-proteins</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Sep 26</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000232094200017</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">4-6</style></number><volume><style face="normal" font="default" size="100%">413</style></volume><pages><style face="normal" font="default" size="100%">335-341</style></pages><isbn><style face="normal" font="default" size="100%">0009-2614</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;We present pressure tuning hole burning experiments on thionin with alpha-cyclodextrin (alpha-CD) and beta-cyclodextrin (beta-CD) in a glycerol/water glass. The low temperature absorption spectra do not show the formation of a caging complex. The pressure tuning data, however, show that the compressibility of the sample with beta-CD, where the formation of an inclusion complex is not restricted due to geometrical reasons increases as compared to the other samples. This is just the opposite of what one would expect. This increase is interpreted in terms of a reduced solvent density around the chromophore due to the hydrophobic effect. (c) 2005 Elsevier B.V. All rights reserved.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000232094200017</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;967MBTimes Cited:3Cited References Count:24&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Friedrich, JTech Univ Munich, Dept Phys E14, D-85350 Freising Weihenstephan, GermanyTech Univ Munich, Dept Phys E14, D-85350 Freising Weihenstephan, GermanyTech Univ Munich, Dept Phys E14, D-85350 Freising Weihenstephan, GermanyTech Univ Munich, Lehrstuhl Phys, D-85350 Freising Weihenstephan, GermanyAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 10764, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Yang, Y. P.</style></author><author><style face="normal" font="default" size="100%">Huang, K. H.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Hecht, C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Investigation of thionin-DNA interaction by satellite hole spectroscopy</style></title><secondary-title><style face="normal" font="default" size="100%">Optics and Spectroscopy</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Opt Spectrosc+</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">9-aminoacridine</style></keyword><keyword><style  face="normal" font="default" size="100%">absorption-spectra</style></keyword><keyword><style  face="normal" font="default" size="100%">acids</style></keyword><keyword><style  face="normal" font="default" size="100%">burning spectra</style></keyword><keyword><style  face="normal" font="default" size="100%">COMPLEXES</style></keyword><keyword><style  face="normal" font="default" size="100%">glasses</style></keyword><keyword><style  face="normal" font="default" size="100%">methylene-blue</style></keyword><keyword><style  face="normal" font="default" size="100%">organic-molecules</style></keyword><keyword><style  face="normal" font="default" size="100%">polynucleotides</style></keyword><keyword><style  face="normal" font="default" size="100%">proteins</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000229951200003</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">5</style></number><volume><style face="normal" font="default" size="100%">98</style></volume><pages><style face="normal" font="default" size="100%">655-660</style></pages><isbn><style face="normal" font="default" size="100%">0030-400X</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The interactions of the two tautomers of thionin dye with DNA have been investigated by using satellite hole burning spectroscopy. Similar features in the absorption and satellite hole spectra of thionin in the presence of calf thymus (CT) DNA and polynucleotides [d(GC)(6)](2) (GC) suggested that thionin preferentially binds to GC rather than polynucleotides [d(AT)(6)](2) (AT). Different binding effects of the two tautomers to DNA could be observed. While the imino form fully intercalates into the DNA base pairs, the amino form is only partially intercalated. In addition, a broad hole associated with an antihole appeared in the presence of DNA, particularly in GC base pairs. The coincidence of the antihole with the absorption band of the amino form showed that the amino form is the photoproduct of the imino form. An increase in intensity of the broad hole and its antihole and the loss of nonresonant hole intensity upon interaction with CT DNA could be described by rapid ground state recovery resulting from fast charge transfer between the intercalated thionin and a guanine base quenching the internal conversion. (c) 2005 Pleiades Publishing, Inc.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000229951200003</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;937ULTimes Cited:2Cited References Count:25&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanTech Univ Munich, Dept Phys, Lehrstuhl Phys, D-85350 Freising Weihenstephan, Germany</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kang, C. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Cheng, J. Y.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Simple method in diagnosing cancer cells by a novel fluorescence probe BMVC</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of the Chinese Chemical Society</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Chin Chem Soc-Taip</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bmvc</style></keyword><keyword><style  face="normal" font="default" size="100%">cell-based microarray</style></keyword><keyword><style  face="normal" font="default" size="100%">detection limit</style></keyword><keyword><style  face="normal" font="default" size="100%">diagnosis of cancer cells</style></keyword><keyword><style  face="normal" font="default" size="100%">led</style></keyword><keyword><style  face="normal" font="default" size="100%">quadruplex DNA</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Dec</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000234444900001</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">6</style></number><volume><style face="normal" font="default" size="100%">52</style></volume><pages><style face="normal" font="default" size="100%">1069-1072</style></pages><isbn><style face="normal" font="default" size="100%">0009-4536</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Different cellular accumulations with distinct fluorescence properties of BMVC in cancer cells from normal cells allow us to establish a simple and economic method for the diagnosis of cancer cells. With using a light emitting diode to excite the BMVC molecule, microarray fluorescence analysis of a cell-based glass chip provides an easy method towards the detection of a limited number of cancer cells.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000234444900001</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;000EYTimes Cited:4Cited References Count:8&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Chang, TCAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, POB 23-166, Taipei 106, TaiwanAcad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, TaiwanAcad Sinica, Res Ctr Appl Sci, Taipei 106, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Kang, C. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Simple method in diagnosing cancer cells by a novel fluorescence probe BMVC</style></title><secondary-title><style face="normal" font="default" size="100%">Abstracts of Papers of the American Chemical Society</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Abstr Pap Am Chem S</style></alt-title></titles><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Aug 28</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://WOS:000236797300460</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">230</style></volume><pages><style face="normal" font="default" size="100%">U241-U241</style></pages><isbn><style face="normal" font="default" size="100%">0065-7727</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">n/a</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000236797300460</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;032TJ137-ANYLTimes Cited:0Cited References Count:0&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Acad Sinica, Inst Atom &amp; Mol Sci, Taipei 106, Taiwan</style></auth-address></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chang, C. C.</style></author><author><style face="normal" font="default" size="100%">Kuo, I. C.</style></author><author><style face="normal" font="default" size="100%">Lin, J. J.</style></author><author><style face="normal" font="default" size="100%">Lu, Y. C.</style></author><author><style face="normal" font="default" size="100%">Chen, C. T.</style></author><author><style face="normal" font="default" size="100%">Back, H. T.</style></author><author><style face="normal" font="default" size="100%">Lou, P. J.</style></author><author><style face="normal" font="default" size="100%">Chang, T. C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A novel carbazole derivative, BMVC: a potential antitumor agent and fluorescence marker of cancer cells</style></title><secondary-title><style face="normal" font="default" size="100%">Chem Biodivers</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Chemistry &amp; biodiversity</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antineoplastic Agents/*chemistry/diagnostic use/pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Biological Markers/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbazoles/*chemistry/diagnostic use/pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line, Tumor</style></keyword><keyword><style  face="normal" font="default" size="100%">Cells, Cultured</style></keyword><keyword><style  face="normal" font="default" size="100%">Drug Screening Assays, Antitumor/methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Drugs, Investigational/chemistry/diagnostic use/pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescent Dyes/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Microscopy, Fluorescence/methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Pyridinium Compounds/*chemistry/diagnostic use/pharmacology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Sep</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17191915</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">9</style></number><edition><style face="normal" font="default" size="100%">2006/12/29</style></edition><volume><style face="normal" font="default" size="100%">1</style></volume><pages><style face="normal" font="default" size="100%">1377-84</style></pages><isbn><style face="normal" font="default" size="100%">1612-1880 (Electronic)1612-1872 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;We have investigated a novel compound, 3,6-bis[2-(1-methylpyridinium)vinyl]carbazole diiodide (BMVC), for inhibiting telomerase activity and distinguishing human lung H1299 and oral Ca9-22 cancer cells from lung IMR90 and skin Detroit-551 normal fibroblast cells. The telomeric repeat amplification protocol (TRAP) assay shows that the concentration of BMVC that inhibits 50% of the telomerase activity (IC50) is ca. 0.05 microM. On the other hand, the cell-viability assay indicates that the cytotoxicity was less than 15% to the H1299 and Ca9-22 cancer cells, and almost negligible to the MRC-5 and Detroit-551 normal cells after incubation with 0.5 microM BMVC for 72 h. The low concentration of 0.05 microM of BMVC can inhibit telomerase activity but does not have general toxic effects to normal cells, implying that BMVC is a promising telomerase inhibitor. Moreover, wide-field fluorescence images of 0.1 microM BMVC-treated cells show bright fluorescence spots in the nuclei of the most H1299 and Ca9-22 cancer cells. Interestingly, similar fluorescence spots are hardly observed in the nuclei of the IMR90 and Detroit-551 normal cells, implying that BMVC might be a useful marker to distinguish tumor cells and normal cells.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Comparative StudyResearch Support, Non-U.S. Gov't</style></work-type><accession-num><style face="normal" font="default" size="100%">17191915</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Chang, Cheng-ChungKuo, I-ChunLin, Jing-JerLu, Yu-ChengChen, Chin-TinBack, Hong-TsunLou, Pei-JenChang, Ta-ChauSwitzerlandChem Biodivers. 2004 Sep;1(9):1377-84.&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei, 106, Taiwan, Republic of China.</style></auth-address></record></records></xml>