@article {568,
	title = {A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress},
	journal = {Nucleic Acids Research},
	volume = {41},
	number = {3},
	year = {2013},
	note = {<p>109FMTimes Cited:0Cited References Count:33</p>
},
	month = {Feb},
	pages = {1533-1543},
	abstract = {<p>We reported that non-targeting siRNA (NT-siRNA) stress induces non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) expression to cooperate with exoribonuclease XRN2 for releasing the stress [Wei,P.C., Lo,W.T., Su,M.I., Shew,J.Y. and Lee, W. H. (2011) Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress. Nucleic Acids Res., 40, 323-332]. However, how NT-siRNA stress inducing NPGPx expression remains elusive. In this communication, we showed that the proximal promoter of NPGPx contained a mixed G-quadruplex (G4) structure, and disrupting the structure diminished NT-siRNA induced NPGPx promoter activity. We also demonstrated that nucleolin (NCL) specifically bonded to the G4-containing sequences to replace the originally bound Sp1 at the NPGPx promoter on NT-siRNA stress. Consistently, overexpression of NCL further increased NPGPx promoter activity, whereas depletion of NCL desensitized NPGPx promoter to NT-siRNA stress. These results suggest that the cis-element with mixed G4 structure at the NPGPx promoter plays an essential role for its transactivation mediated by NCL to release cells from NT-siRNA stress.</p>
},
	keywords = {c-myc, complex-formation, DNA structures, gene, initiation, myc g-quadruplex, protein, region, telomeric DNA, transcription},
	isbn = {0305-1048},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/23241391},
	author = {Wei, P. C. and Wang, Z. F. and Lo, W. T. and Su, M. I. and Shew, J. Y. and Chang, T. C. and Lee, W. H.}
}
