@article {557,
	title = {Emulsified BMVC derivative induced filtration for G-quadruplex DNA structural separation},
	journal = {Nucleic Acids Res},
	volume = {39},
	number = {17},
	year = {2011},
	note = {<p>Tsai, Yu-LinWang, Zi-FuChen, Wei-WenChang, Ta-ChauEnglandNucleic Acids Res. 2011 Sep 1;39(17):e114. doi: 10.1093/nar/gkr499. Epub 2011 Jun 28.</p>
},
	month = {Sep 1},
	pages = {e114},
	type = {Research Support, Non-U.S. Gov{\textquoteright}t},
	edition = {2011/07/01},
	abstract = {<p>A novel method based on emulsion/filtration is introduced for G-quadruplex DNA structural separation. We first synthesized a lipophilic analogue of BMVC, 3,6-Bis(1-methyl-4-vinylpyridinium)-9-(12{\textquoteright}-bromododecyl) carbazole diiodide (BMVC-12C-Br), which can form an oil-in-water (o/w) phase emulsion. Due to the binding preferences of BMVC-12C-Br emulsion to some specific DNA structures, the large emulsion ( approximately 2 microm) bound DNA was separated from the small free DNA in the filtrate by a 0.22 microm pore size MCE membrane. This method is able to isolate the non-parallel G-quadruplexes from the parallel G-quadruplexes and the linear duplexes from both G-quadruplexes. In addition, this method allows us not only to determine the absence of the parallel G-quadruplexes of d(T(2)AG(3))(4) and the presence of the parallel G-quadruplexes of d(T(2)AG(3))(2) in K(+) solution, but also to verify structural conversion from antiparallel to parallel G-quadruplexes of d[AG(3)(T(2)AG(3))(3)] in K(+) solution under molecular PEG condition. Moreover, this emulsion can separate the non-parallel G-quadruplexes of d(G(3)CGCG(3)AGGAAG(5)CG(3)) monomer from the parallel G-quadruplexes of its dimer in K(+) solution. Together with NMR spectra, one can simplify the spectra for both the free DNA and the bound DNA to establish a spectrum-structure correlation for further structural analysis.</p>
},
	keywords = {*G-Quadruplexes, Carbazoles/*chemistry, DNA/*chemistry/*isolation \& purification, Emulsions, Filtration/*methods, Fluorescence, Fluorescent Dyes/*chemistry, Hydrocarbons, Brominated/*chemistry, Microscopy, Fluorescence, Potassium/chemistry, Pyridinium Compounds/*chemistry},
	isbn = {1362-4962 (Electronic)0305-1048 (Linking)},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/21715373},
	author = {Tsai, Y. L. and Wang, Z. F. and Chen, W. W. and Chang, T. C.}
}
