<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Yao, Y.-J.</style></author><author><style face="normal" font="default" size="100%">Lin, K.-C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">DNA interaction probed by evanescent wave cavity ring-down absorption spectroscopy via functionalized gold nanoparticles</style></title><secondary-title><style face="normal" font="default" size="100%">Analytica Chimica Acta</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Absorption spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">Adsorption isotherms</style></keyword><keyword><style  face="normal" font="default" size="100%">Article</style></keyword><keyword><style  face="normal" font="default" size="100%">binding affinity</style></keyword><keyword><style  face="normal" font="default" size="100%">binding assay</style></keyword><keyword><style  face="normal" font="default" size="100%">binding kinetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Cavity ring-down</style></keyword><keyword><style  face="normal" font="default" size="100%">complementary DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA binding</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA denaturation</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA determination</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA hybridization</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA interaction</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA sensing</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA strand</style></keyword><keyword><style  face="normal" font="default" size="100%">Electromagnetic wave reflection</style></keyword><keyword><style  face="normal" font="default" size="100%">equilibrium constant</style></keyword><keyword><style  face="normal" font="default" size="100%">Equilibrium constants</style></keyword><keyword><style  face="normal" font="default" size="100%">evanescent wave cavity ring down absorption spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">GOLD</style></keyword><keyword><style  face="normal" font="default" size="100%">Gold alloys</style></keyword><keyword><style  face="normal" font="default" size="100%">gold nanoparticle</style></keyword><keyword><style  face="normal" font="default" size="100%">Gold nanoparticles</style></keyword><keyword><style  face="normal" font="default" size="100%">hemoglobin</style></keyword><keyword><style  face="normal" font="default" size="100%">hemoglobin gene</style></keyword><keyword><style  face="normal" font="default" size="100%">hybridization</style></keyword><keyword><style  face="normal" font="default" size="100%">ionic strength</style></keyword><keyword><style  face="normal" font="default" size="100%">limit of detection</style></keyword><keyword><style  face="normal" font="default" size="100%">Metal nanoparticles</style></keyword><keyword><style  face="normal" font="default" size="100%">pH</style></keyword><keyword><style  face="normal" font="default" size="100%">priority journal</style></keyword><keyword><style  face="normal" font="default" size="100%">sandwich hybridization assay</style></keyword><keyword><style  face="normal" font="default" size="100%">sickle cell anemia</style></keyword><keyword><style  face="normal" font="default" size="100%">Sickle cell disease</style></keyword><keyword><style  face="normal" font="default" size="100%">silicon dioxide</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.scopus.com/inward/record.uri?eid=2-s2.0-84898646295&amp;doi=10.1016%2fj.aca.2014.01.045&amp;partnerID=40&amp;md5=ecc7a82cdcb626321f22c277ad27a307</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">820</style></volume><pages><style face="normal" font="default" size="100%">1-8</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Evanescent wave cavity ring-down absorption spectroscopy (EW-CRDS) is employed to study interaction and binding kinetics of DNA strands by using gold nanoparticles (Au NPs) as sensitive reporters. These Au NPs are connected to target DNA of study that hybridizes with the complementary DNA fixed on the silica surface. By the absorbance of Au NPs, the interaction between two DNA strands may be examined to yield an adsorption equilibrium constant of 2.2×1010M-1 using Langmuir fit. The binding efficiency that is affected by ion concentration, buffer pH and temperature is also examined. This approach is then applied to the label-free detection of the DNA mutation diseases using the sandwich hybridization assay. For monitoring a gene associated with sickle-cell anemia, the detection limit and the adsorption equilibrium constant is determined to be 1.2pM and (3.7±0.8)×1010M-1, distinct difference from the perfectly matched DNA sequence that yields the corresponding 0.5pM and (1.1±0.2)×1011M-1. The EW-CRDS method appears to have great potential for the investigation of the kinetics of a wide range of biological reactions. © 2014 Elsevier B.V.&lt;/p&gt;
</style></abstract><notes><style face="normal" font="default" size="100%">&lt;p&gt;cited By 1&lt;/p&gt;
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