<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dhenadhayalan, N.</style></author><author><style face="normal" font="default" size="100%">K. Yadav</style></author><author><style face="normal" font="default" size="100%">Sriram, M.I.</style></author><author><style face="normal" font="default" size="100%">Lee, H.-L.</style></author><author><style face="normal" font="default" size="100%">Lin, K.-C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Ultra-sensitive DNA sensing of a prostate-specific antigen based on 2D nanosheets in live cells</style></title><secondary-title><style face="normal" font="default" size="100%">Nanoscale</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antigens</style></keyword><keyword><style  face="normal" font="default" size="100%">Biocompatibility</style></keyword><keyword><style  face="normal" font="default" size="100%">Biosensing Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Confocal</style></keyword><keyword><style  face="normal" font="default" size="100%">Confocal fluorescence microscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">confocal microscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">Detection limits</style></keyword><keyword><style  face="normal" font="default" size="100%">Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Energy transfer</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescence microscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescence quenching</style></keyword><keyword><style  face="normal" font="default" size="100%">genetic procedures</style></keyword><keyword><style  face="normal" font="default" size="100%">HEK293 cell line</style></keyword><keyword><style  face="normal" font="default" size="100%">HEK293 Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">human</style></keyword><keyword><style  face="normal" font="default" size="100%">Human embryonic kidneys</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">limit of detection</style></keyword><keyword><style  face="normal" font="default" size="100%">male</style></keyword><keyword><style  face="normal" font="default" size="100%">MICROSCOPY</style></keyword><keyword><style  face="normal" font="default" size="100%">molybdenum</style></keyword><keyword><style  face="normal" font="default" size="100%">Molybdenum oxide</style></keyword><keyword><style  face="normal" font="default" size="100%">nanomaterial</style></keyword><keyword><style  face="normal" font="default" size="100%">NANOSHEETS</style></keyword><keyword><style  face="normal" font="default" size="100%">NANOSTRUCTURES</style></keyword><keyword><style  face="normal" font="default" size="100%">Prostate cancers</style></keyword><keyword><style  face="normal" font="default" size="100%">Prostate specific antigen</style></keyword><keyword><style  face="normal" font="default" size="100%">prostate tumor</style></keyword><keyword><style  face="normal" font="default" size="100%">Prostate-Specific Antigen</style></keyword><keyword><style  face="normal" font="default" size="100%">Prostatic Neoplasms</style></keyword><keyword><style  face="normal" font="default" size="100%">Quenching</style></keyword><keyword><style  face="normal" font="default" size="100%">Sensing platforms</style></keyword><keyword><style  face="normal" font="default" size="100%">Two dimensional nanosheets</style></keyword><keyword><style  face="normal" font="default" size="100%">Urology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028440294&amp;doi=10.1039%2fc7nr03431h&amp;partnerID=40&amp;md5=eb3233a26be749539cc67e16a9e1aa54</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">33</style></number><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">12087-12095</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Herein, we report ultra-sensitive sensing of a prostate-specific antigen (PSA), which is used as a biomarker to detect prostate cancer, using a molybdenum series (MoO3, MoS2, and MoSe2) of two-dimensional nanosheets (2D NSs). Moreover, the design of a 2D NS-based PSA aptamer sensor system was demonstrated based on a fluorescence turn-on mechanism in the presence of a target. The 2D NSs acted as an excellent sensing platform in which the PSA aptamer was adsorbed on the NSs and subsequent energy transfer between them led to fluorescence quenching of the aptamer. The detection limit of PSA was achieved to be 13 pM for MoO3 NSs, whereas the MoS2 and MoSe2 systems exhibited a detection limit of 72 and 157 pM, respectively. To the best of our knowledge, this is the first report on the ultra-sensitive detection of a 2D NS-based aptamer sensor. The in vitro bioimaging measurements were performed using confocal fluorescence microscopy. Herein, PSA detection was successfully demonstrated in human embryonic kidney 293T (HEK) live cells. Moreover, the MoO3, MoS2, and MoSe2 NSs exhibit excellent biocompatibility and low toxicity; thus, these 2D NSs can be used as a promising sensor platform to detect prostate cancer. This journal is © The Royal Society of Chemistry.&lt;/p&gt;
</style></abstract><notes><style face="normal" font="default" size="100%">&lt;p&gt;cited By 4&lt;/p&gt;
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