@article {Dhenadhayalan201712087,
	title = {Ultra-sensitive DNA sensing of a prostate-specific antigen based on 2D nanosheets in live cells},
	journal = {Nanoscale},
	volume = {9},
	number = {33},
	year = {2017},
	note = {<p>cited By 4</p>
},
	pages = {12087-12095},
	abstract = {<p>Herein, we report ultra-sensitive sensing of a prostate-specific antigen (PSA), which is used as a biomarker to detect prostate cancer, using a molybdenum series (MoO3, MoS2, and MoSe2) of two-dimensional nanosheets (2D NSs). Moreover, the design of a 2D NS-based PSA aptamer sensor system was demonstrated based on a fluorescence turn-on mechanism in the presence of a target. The 2D NSs acted as an excellent sensing platform in which the PSA aptamer was adsorbed on the NSs and subsequent energy transfer between them led to fluorescence quenching of the aptamer. The detection limit of PSA was achieved to be 13 pM for MoO3 NSs, whereas the MoS2 and MoSe2 systems exhibited a detection limit of 72 and 157 pM, respectively. To the best of our knowledge, this is the first report on the ultra-sensitive detection of a 2D NS-based aptamer sensor. The in vitro bioimaging measurements were performed using confocal fluorescence microscopy. Herein, PSA detection was successfully demonstrated in human embryonic kidney 293T (HEK) live cells. Moreover, the MoO3, MoS2, and MoSe2 NSs exhibit excellent biocompatibility and low toxicity; thus, these 2D NSs can be used as a promising sensor platform to detect prostate cancer. This journal is {\textcopyright} The Royal Society of Chemistry.</p>
},
	keywords = {Antigens, Biocompatibility, Biosensing Techniques, Chemistry, Confocal, Confocal fluorescence microscopy, confocal microscopy, Detection limits, Diseases, DNA, Energy transfer, Fluorescence, Fluorescence microscopy, Fluorescence quenching, genetic procedures, HEK293 cell line, HEK293 Cells, human, Human embryonic kidneys, Humans, limit of detection, male, MICROSCOPY, molybdenum, Molybdenum oxide, nanomaterial, NANOSHEETS, NANOSTRUCTURES, Prostate cancers, Prostate specific antigen, prostate tumor, Prostate-Specific Antigen, Prostatic Neoplasms, Quenching, Sensing platforms, Two dimensional nanosheets, Urology},
	doi = {10.1039/c7nr03431h},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028440294\&doi=10.1039\%2fc7nr03431h\&partnerID=40\&md5=eb3233a26be749539cc67e16a9e1aa54},
	author = {Dhenadhayalan, N. and K. Yadav and Sriram, M.I. and Lee, H.-L. and Lin, K.-C.}
}
